PMID- 9784544
OWN - NLM
STAT- MEDLINE
DCOM- 19981123
LR  - 20240206
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 66
IP  - 11
DP  - 1998 Nov
TI  - Helicobacter pylori lipopolysaccharide binds to CD14 and stimulates release of 
      interleukin-8, epithelial neutrophil-activating peptide 78, and monocyte 
      chemotactic protein 1 by human monocytes.
PG  - 5357-63
AB  - Helicobacter pylori gastritis is characterized by leukocyte infiltration of the 
      gastric mucosa. The aims of this study were to determine whether H. 
      pylori-derived factors stimulate chemokine release from human monocytes and to 
      ascertain whether H. pylori lipopolysaccharide (LPS) may be responsible for this 
      effect. Human peripheral blood monocytes were exposed to an H. pylori water 
      extract (HPE) or to purified H. pylori LPS. Levels of the chemokines 
      interleukin-8 (IL-8), epithelial neutrophil-activating peptide 78 (ENA-78), and 
      monocyte chemotactic protein 1 (MCP-1) were measured by enzyme-linked 
      immunosorbent assay. The contribution of H. pylori LPS to monocyte activation was 
      determined by using the LPS antagonist Rhodobacter sphaeroides lipid A (RSLA) and 
      a blocking monoclonal antibody to CD14 (60bca). HPE increased monocyte secretion 
      of IL-8, ENA-78, and MCP-1. Heat treatment of HPE did not reduce its ability to 
      activate monocytes. Purified H. pylori LPS also stimulated monocyte chemokine 
      production but was 1,000-fold less potent than Salmonella minnesota lipid A. RSLA 
      blocked H. pylori LPS-induced monocyte IL-8 release in a dose-dependent fashion 
      (maximal inhibition 82%, P < 0.001). RSLA also inhibited HPE-induced IL-8 release 
      (by 93%, P < 0.001). The anti-CD14 monoclonal antibody 60bca substantially 
      inhibited IL-8 release from HPE-stimulated monocytes (by 88%, P < 0.01), whereas 
      the nonblocking anti-CD14 monoclonal antibody did not. These experiments with 
      potent and specific LPS inhibitors indicate that the main monocyte-stimulating 
      factor in HPE is LPS. H. pylori LPS, acting through CD14, stimulates human 
      monocytes to release the neutrophil-activating chemokines IL-8 and ENA-78 and the 
      monocyte-activating chemokine MCP-1. Despite its low relative potency, H. pylori 
      LPS may play an important role in the pathogenesis of H. pylori gastritis.
FAU - Bliss, C M Jr
AU  - Bliss CM Jr
AD  - Section of Gastroenterology, Boston Medical Center, Boston University School of 
      Medicine, Boston, Massachusetts 02118, USA.
FAU - Golenbock, D T
AU  - Golenbock DT
FAU - Keates, S
AU  - Keates S
FAU - Linevsky, J K
AU  - Linevsky JK
FAU - Kelly, C P
AU  - Kelly CP
LA  - eng
GR  - GM54060/GM/NIGMS NIH HHS/United States
GR  - R01 GM054060/GM/NIGMS NIH HHS/United States
GR  - DK02128/DK/NIDDK NIH HHS/United States
GR  - DK54920/DK/NIDDK NIH HHS/United States
GR  - R37 GM054060/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (CXCL5 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL5)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipid A)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (endotoxin receptor)
SB  - IM
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CXCL5
MH  - *Chemokines, CXC
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Interleukin-8/*analogs & derivatives/antagonists & 
      inhibitors/biosynthesis/*metabolism
MH  - Lipid A/pharmacology
MH  - Lipopolysaccharide Receptors/*metabolism
MH  - Lipopolysaccharides/antagonists & inhibitors/*metabolism/*pharmacology
MH  - Monocytes/drug effects/*immunology/metabolism
MH  - Receptors, Immunologic/metabolism/physiology
MH  - Rhodobacter sphaeroides
MH  - Up-Regulation/drug effects/immunology
PMC - PMC108670
EDAT- 1998/10/24 02:14
MHDA- 2001/03/28 10:01
PMCR- 1998/11/01
CRDT- 1998/10/24 02:14
PHST- 1998/10/24 02:14 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/10/24 02:14 [entrez]
PHST- 1998/11/01 00:00 [pmc-release]
AID - 0023 [pii]
AID - 10.1128/IAI.66.11.5357-5363.1998 [doi]
PST - ppublish
SO  - Infect Immun. 1998 Nov;66(11):5357-63. doi: 10.1128/IAI.66.11.5357-5363.1998.