PMID- 9787172
OWN - NLM
STAT- MEDLINE
DCOM- 19981130
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 92
IP  - 9
DP  - 1998 Nov 1
TI  - Alloantigen-stimulated anti-HIV activity.
PG  - 3346-54
AB  - A number of studies have suggested that an immune response to human leukocyte 
      antigen (HLA) alloantigens may contribute to protection against HIV infection. In 
      the present study, we examined the effect of alloantigen-stimulated cell lines 
      obtained from peripheral blood mononuclear cells (PBMC) of HIV-uninfected (HIV-) 
      individuals and the soluble factors produced by these cell lines on HIV-1 
      replication. Multiple in vitro restimulation with irradiated allogeneic PBMC from 
      HIV- donors resulted in the expansion of CD8(+) T-cell lines that inhibited HIV-1 
      replication when cocultured with either autologous or heterologous in 
      vitro-infected phytohemagglutinin (PHA) blasts. Supernatants from the 
      alloantigen-stimulated cell lines also inhibited HIV replication in both PHA 
      blasts and a chronically infected cell line. The alloantigen-stimulated cell 
      lines and the factors they produced inhibited both T-cell-tropic (T) and 
      macrophage-tropic (M) isolates of HIV-1. Blocking experiments using 
      anti-chemokine antibodies suggested that this inhibition of HIV replication was 
      not due to the beta-chemokines present in cocultures of cell lines with 
      HIV-infected blasts. These results indicate that alloantigen-stimulation of PBMC 
      from HIV- individuals activates CD8(+) T cells that produce soluble factor(s) 
      that inhibit HIV replication of a wide spectrum of HIV-1 isolates through a 
      chemokine-independent mechanism. This is a US government work. There are no 
      restrictions on its use.
FAU - Pinto, L A
AU  - Pinto LA
AD  - Experimental Immunology Branch and Laboratory of Tumor Cell Biology, National 
      Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
FAU - Sharpe, S
AU  - Sharpe S
FAU - Cohen, D I
AU  - Cohen DI
FAU - Shearer, G M
AU  - Shearer GM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biological Factors)
RN  - 0 (Chemokines)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Isoantigens)
RN  - 0 (Lymphokines)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Receptors, HIV)
SB  - IM
CIN - Blood. 2000 Mar 1;95(5):1875-6. PMID: 10744387
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Biological Factors/isolation & purification/metabolism/*pharmacology
MH  - CD8-Positive T-Lymphocytes/immunology/*metabolism
MH  - Cell Line
MH  - Chemokines/antagonists & inhibitors/physiology
MH  - Culture Media, Conditioned/pharmacology
MH  - HIV-1/classification/*drug effects/physiology
MH  - Histocompatibility
MH  - Humans
MH  - Isoantigens/*immunology
MH  - Leukocytes, Mononuclear/immunology/*metabolism
MH  - *Lymphocyte Activation
MH  - Lymphokines/analysis/antagonists & inhibitors/immunology
MH  - Macrophages/virology
MH  - Phytohemagglutinins/pharmacology
MH  - Receptors, HIV/physiology
MH  - T-Lymphocytes/virology
MH  - Virus Replication/*drug effects
EDAT- 1998/10/27 00:00
MHDA- 1998/10/27 00:01
CRDT- 1998/10/27 00:00
PHST- 1998/10/27 00:00 [pubmed]
PHST- 1998/10/27 00:01 [medline]
PHST- 1998/10/27 00:00 [entrez]
AID - S0006-4971(20)57885-3 [pii]
PST - ppublish
SO  - Blood. 1998 Nov 1;92(9):3346-54.