PMID- 9787654
OWN - NLM
STAT- MEDLINE
DCOM- 19981105
LR  - 20220317
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 143
IP  - 9
DP  - 1998
TI  - Sequence analysis of porcine reproductive and respiratory syndrome virus of the 
      American type collected from Danish swine herds.
PG  - 1683-700
AB  - Vaccine-like viruses of American type of porcine reproductive and respiratory 
      syndrome virus (PRRSV) were detected in serum samples by RT-PCR. The viruses were 
      analysed by nucleotide sequencing of the genomic region encoding open reading 
      frames 2 to 7. During the ongoing study of Danish isolates of PRRSV by means of 
      nucleotide sequencing, RT-PCR reactions and subsequent nucleotide sequencing 
      showed the presence of American type PRRSV in Danish breeding herds. Most likely, 
      these atypical viruses originated from boars vaccinated with live vaccine of 
      American type (MLV RespPRRS), which were taken to artificial insemination centres 
      and there brought together with unvaccinated boars already at the centres. The 
      nucleotide sequences of three Danish viruses of American type PRRSV were compared 
      to those of known PRRSV isolates. The nucleotide sequence identities of the 
      atypical Danish isolates were between 99.2-99.5% to the vaccine virus RespPRRS 
      and 99.0-99.3% to VR2332 which are the parental virus to the vaccine virus. 
      Phylogenetic analysis including field isolates of American type supports the 
      conclusion that the introduction of American type PRRSV in Denmark was due to 
      spread of vaccine virus.
FAU - Madsen, K G
AU  - Madsen KG
AD  - Danish Veterinary Institute for Virus Research Lindholm, Kalvehave, Denmark.
FAU - Hansen, C M
AU  - Hansen CM
FAU - Madsen, E S
AU  - Madsen ES
FAU - Strandbygaard, B
AU  - Strandbygaard B
FAU - Botner, A
AU  - Botner A
FAU - Sorensen, K J
AU  - Sorensen KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/immunology
MH  - Swine
MH  - Viral Vaccines/immunology
EDAT- 1998/10/27 00:00
MHDA- 1998/10/27 00:01
CRDT- 1998/10/27 00:00
PHST- 1998/10/27 00:00 [pubmed]
PHST- 1998/10/27 00:01 [medline]
PHST- 1998/10/27 00:00 [entrez]
AID - 10.1007/s007050050409 [doi]
PST - ppublish
SO  - Arch Virol. 1998;143(9):1683-700. doi: 10.1007/s007050050409.