PMID- 9788673
OWN - NLM
STAT- MEDLINE
DCOM- 19981221
LR  - 20151119
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 14
IP  - 15
DP  - 1998 Oct 10
TI  - Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 
      16: biologic activity and effect on simian immunodeficiency virus infection 
      and/or replication.
PG  - 1323-8
AB  - Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through 
      inhibition of HIV and SIV mRNA transcription. To evaluate its role further, we 
      compared IL-16 cloned from disease-susceptible rhesus macaques and 
      disease-resistant sooty mangabeys. Recombinant rhesus macaque (rr) IL-16 was 
      compared with recombinant sooty mangabey (rm), human, and other nonhuman primate 
      IL-16 sequences and evaluated for its ability to induce chemotaxis and inhibit 
      the mixed lymphocyte response (MLR). Also, rrIL-16 and rmIL-16 were evaluated for 
      suppression of SIVmac251, which replicates efficiently in T cells and 
      monocyte/macrophages (dual tropic), and cloned SIVmac239, which replicates 
      efficiently in T cells (T tropic). Sequence comparison of rrIL-16 and rmIL-16 
      with human IL-16 showed >97% amino acid identity. Biocharacterization of rrIL-16 
      revealed potent induction of chemotaxis (p < 0.05) and marked inhibition of MLR 
      (73 +/- 0.6%,p < 0.05) in rhesus and human cell systems. Using rrIL-16 and 
      rmIL-16, p27 antigen production from PBMCs infected with SIVmac251 was decreased 
      up to 70% (p < 0.05 and p < 0.01, respectively). In similar cultures infected 
      with SIVmac239, rrIL-16 and rmIL-16 reduced p27 levels by 96 and 100%, 
      respectively. These data demonstrate the biologic and antiviral functionality of 
      rrIL-16 and rmIL-16.
FAU - Lee, M E
AU  - Lee ME
AD  - Department of Pathology and Laboratory Medicine and the Winship Cancer Center, 
      Emory University School of Medicine, Atlanta, Georgia 30329, USA.
FAU - Adams, J W
AU  - Adams JW
FAU - Villinger, F
AU  - Villinger F
FAU - Brar, S S
AU  - Brar SS
FAU - Meadows, M
AU  - Meadows M
FAU - Bucur, S Z
AU  - Bucur SZ
FAU - Lackey, D A 3rd
AU  - Lackey DA 3rd
FAU - Brice, G T
AU  - Brice GT
FAU - Cruikshank, W W
AU  - Cruikshank WW
FAU - Ansari, A A
AU  - Ansari AA
FAU - Hillyer, C D
AU  - Hillyer CD
LA  - eng
GR  - HL53733/HL/NHLBI NIH HHS/United States
GR  - HL55176/HL/NHLBI NIH HHS/United States
GR  - HL55176-02S1/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Interleukin-16)
RN  - 0 (Recombinant Proteins)
SB  - IM
EIN - AIDS Res Hum Retroviruses 1998 Nov 20;14(17):1604
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Cercocebus atys
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Cloning, Molecular
MH  - Humans
MH  - Interleukin-16/*genetics/pharmacology
MH  - Lymphocyte Culture Test, Mixed
MH  - Macaca mulatta
MH  - Macaca nemestrina
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/genetics/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Simian Acquired Immunodeficiency Syndrome/*drug therapy
MH  - Simian Immunodeficiency Virus/*drug effects
MH  - T-Lymphocytes/cytology/drug effects
MH  - Virus Replication/drug effects
EDAT- 1998/10/27 03:03
MHDA- 2001/03/28 10:01
CRDT- 1998/10/27 03:03
PHST- 1998/10/27 03:03 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/10/27 03:03 [entrez]
AID - 10.1089/aid.1998.14.1323 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1998 Oct 10;14(15):1323-8. doi: 
      10.1089/aid.1998.14.1323.