PMID- 9789056
OWN - NLM
STAT- MEDLINE
DCOM- 19981124
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 95
IP  - 22
DP  - 1998 Oct 27
TI  - Quantitative analysis of the immunopotency of genetically transfected dendritic 
      cells.
PG  - 13147-52
AB  - Dendritic cells (DCs) instruct and activate a naive immune system to mount a 
      response toward foreign proteins. Therefore, it has been hypothesized that an 
      ideal vaccine strategy would be to directly introduce genes encoding antigens 
      into DCs. To test this strategy quantitatively, we have compared the immune 
      response elicited by a genetically transfected DC line to that induced by a 
      fibroblast line, or standard genetic immunization. We observe that a single 
      injection of 500-1,000 transfected DCs can produce a response comparable to that 
      of standard genetic immunization, whereas fibroblasts, with up to 50-fold greater 
      transfection efficiency, were less potent. We conclude that transfection of a 
      small number of DCs is sufficient to initiate a wide variety of immune responses. 
      These results indicate that targeting genes to DCs will be important for 
      controlling and augmenting the immunological outcome in genetic immunization.
FAU - Timares, L
AU  - Timares L
AD  - Department of Medicine, University of Texas, Southwestern Medical Center, Dallas, 
      TX 75235-8573, USA.
FAU - Takashima, A
AU  - Takashima A
FAU - Johnston, S A
AU  - Johnston SA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (alpha 1-Antitrypsin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigen-Presenting Cells/immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Fibroblasts/immunology
MH  - Green Fluorescent Proteins
MH  - Immunization/methods
MH  - Luciferases/biosynthesis/genetics
MH  - Luminescent Proteins/biosynthesis/genetics
MH  - *Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred A
MH  - Recombinant Proteins/biosynthesis
MH  - T-Lymphocytes/*immunology
MH  - Transfection/*immunology
MH  - alpha 1-Antitrypsin/biosynthesis/genetics
PMC - PMC23740
EDAT- 1998/10/28 00:00
MHDA- 1998/10/28 00:01
PMCR- 1999/04/27
CRDT- 1998/10/28 00:00
PHST- 1998/10/28 00:00 [pubmed]
PHST- 1998/10/28 00:01 [medline]
PHST- 1998/10/28 00:00 [entrez]
PHST- 1999/04/27 00:00 [pmc-release]
AID - 3261 [pii]
AID - 10.1073/pnas.95.22.13147 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13147-52. doi: 
      10.1073/pnas.95.22.13147.