PMID- 9790508
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20091119
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 23
IP  - 3
DP  - 1998 Nov
TI  - COL1A1-PDGFB fusion in a ring chromosome 4 found in a dermatofibrosarcoma 
      protuberans.
PG  - 263-6
AB  - Dermatofibrosarcoma protuberans (DP), an infiltrative skin tumor of intermediate 
      malignancy, presents specific cytogenetic features such as reciprocal 
      translocations t(17;22)(q22;q13.1) or, more often, supernumerary ring chromosomes 
      derived from t(17;22). Different translocations, including t(2;17) and t(X;7), 
      have also been described. We have shown previously that both r(17;22) and 
      t(17;22) present the same molecular rearrangement fusing the COL1A1 gene on 
      chromosome 17 and the PDGFB gene on chromosome 22. Out of our series of 16 DPs, 
      we detected an extra ring chromosome in tumor T96-1175, which juxtaposed 
      sequences from chromosomes 4 and 17. As shown by fluorescence in situ 
      hybridization (FISH) using chromosome painting and alpha-satellite probes, 
      T96-1175 apparently lacked chromosome 22 material in the ring. However, 
      involvement of chromosome 22 through a rearrangement of PDGFB was shown by 
      Southern blotting, reverse transcriptase-polymerase chain reaction (RT-PCR), and 
      FISH. This study demonstrates that a cryptic molecular rearrangement between 
      chromosomes 17 and 22 occurred in addition to the recombination of chromosomes 4 
      and 17 initially identified by FISH. Assessment for cryptic molecular events 
      should be performed in other variant DP rearrangements.
FAU - Navarro, M
AU  - Navarro M
AD  - Laboratoire de Genetique Chromosomique des Tumeurs, UMR 4569, Faculte de 
      Medecine, Nice, France.
FAU - Simon, M P
AU  - Simon MP
FAU - Migeon, C
AU  - Migeon C
FAU - Turc-Carel, C
AU  - Turc-Carel C
FAU - Pedeutour, F
AU  - Pedeutour F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Chromosome Banding
MH  - Collagen/*genetics
MH  - Dermatofibrosarcoma/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Platelet-Derived Growth Factor/*genetics
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-sis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Ring Chromosomes
MH  - Translocation, Genetic/genetics
EDAT- 1998/10/28 03:03
MHDA- 2000/06/20 09:00
CRDT- 1998/10/28 03:03
PHST- 1998/10/28 03:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/10/28 03:03 [entrez]
AID - 10.1002/(SICI)1098-2264(199811)23:3<263::AID-GCC9>3.0.CO;2-# [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Nov;23(3):263-6.