PMID- 9790727
OWN - NLM
STAT- MEDLINE
DCOM- 19981124
LR  - 20171116
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 189
IP  - 2
DP  - 1998 Nov 1
TI  - Constitutive and induced IL-18 receptor expression by various peripheral blood 
      cell subsets as determined by anti-hIL-18R monoclonal antibody.
PG  - 135-43
AB  - Interleukin-18 (IL-18) was identified as a molecule that induces IFN-gamma 
      production and enhances NK cell cytotoxicity. Characterization of the receptor 
      for human IL-18 (hIL-18R) is important for investigating the physiological role 
      of IL-18 in nature. In the present study, we describe a monoclonal antibody (mAb) 
      against hIL-18R (mAb No. 117-10C). This mAb inhibited the binding of 125I-labeled 
      hIL-18 to IL-18R-expressing L428 cells. This mAb also neutralized hIL-18-induced 
      T helper 1 type cytokine (IFN-gamma and GM-CSF) production by Con A-stimulated 
      PBMC. PBMC were examined for the expression of IL-18R by two-color flow 
      cytometry. Most CD19(+) B cells and a percentage of CD8(+) T cells were found to 
      constitutively express IL-18R. Treatment of PBMC with IL-12 preferentially 
      induced IL-18R expression on CD56(+) NK cells regardless of costimulation with 
      mitogen. IL-18R expression on CD4(+) T cells was induced weakly by IL-12 
      treatment and moderately by PHA stimulation. However, neither could IL-12 
      treatment nor PHA stimulation induce IL-18R expression on CD8(+) T cells. 
      Costimulation with both IL-12 and PHA was necessary for optimal IL-18R expression 
      on CD8(+) T cells as well as on CD56(+) NK cells, CD4(+) T cells, and CD19(+) B 
      cells. These results support the growing number of reports showing that IL-18 has 
      modulatory effects on T, B, and NK cells.
CI  - Copyright 1998 Academic Press.
FAU - Kunikata, T
AU  - Kunikata T
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, 
      702-8006, Japan.
FAU - Torigoe, K
AU  - Torigoe K
FAU - Ushio, S
AU  - Ushio S
FAU - Okura, T
AU  - Okura T
FAU - Ushio, C
AU  - Ushio C
FAU - Yamauchi, H
AU  - Yamauchi H
FAU - Ikeda, M
AU  - Ikeda M
FAU - Ikegami, H
AU  - Ikegami H
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD56 Antigen)
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - CD56 Antigen/analysis
MH  - COS Cells
MH  - Cells, Cultured
MH  - Humans
MH  - Interleukin-12/pharmacology
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Killer Cells, Natural/chemistry
MH  - Leukocytes, Mononuclear/*chemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, Interleukin/*analysis/immunology
MH  - Receptors, Interleukin-18
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - S0008-8749(98)91376-4 [pii]
AID - 10.1006/cimm.1998.1376 [doi]
PST - ppublish
SO  - Cell Immunol. 1998 Nov 1;189(2):135-43. doi: 10.1006/cimm.1998.1376.