PMID- 9790904
OWN - NLM
STAT- MEDLINE
DCOM- 19981123
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 251
IP  - 1
DP  - 1998 Oct 9
TI  - Cloning of the murine interferon-inducible protein 10 (IP-10) receptor and its 
      specific expression in lymphoid organs.
PG  - 41-8
AB  - To isolate the interferon-inducible protein 10 (IP-10) receptor gene, we searched 
      for cells that respond to IP-10. Among several human and murine T cell lines, 
      only CTLL2 cells ( a murine cytotoxic T cell line) responded to IP-10 with 
      transient elevation of intracellular Ca2+. The murine IP-10 receptor gene has 
      been cloned from cDNA derived from CTLL2 cells using the reverse 
      transcriptase-polymerase chain reaction protocol with two degenerate primers 
      corresponding to conserved regions of chemokine receptors. The cDNA encoding the 
      murine IP-10 receptor has an open reading frame of 1101 bp corresponding to a 
      protein of 367 amino acids that exhibits 86 % identity with the human IP-10 
      receptor. It mediates Ca2+ mobilization in response to IP-10, but does not 
      recognize other rodent chemokines, including GRO, RANTES, monocyte 
      chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-1alpha 
      (MIP-1alpha). Northern blot analysis revealed that murine IP-10 and its receptor 
      mRNA were constitutively expressed in the spleen and thymus from normal mouse, 
      while IP-10 and its receptor mRNA were derived from stromal cells and lymphocytes 
      in both tissues, respectively. In vivo treatment with concanavalin A (Con A) for 
      12 hrs revealed that splenocytes significantly induce IP-10 receptor mRNA 
      expression and show a good chemotactic response to IP-10. Therefore, it is 
      supposed that IP-10 and its receptor are important for lymphocyte trafficking to 
      lymphoid organs and that the IP-10 receptor on lymphocytes is rapidly inducible 
      on inflammation or in immunological events.
CI  - Copyright 1998 Academic Press.
FAU - Tamaru, M
AU  - Tamaru M
AD  - JT Central Pharmaceutical Research Institute, Yokohama, Kanagawa, 236-0004, 
      Japan.
FAU - Tominaga, Y
AU  - Tominaga Y
FAU - Yatsunami, K
AU  - Yatsunami K
FAU - Narumi, S
AU  - Narumi S
LA  - eng
SI  - GENBANK/AB003174
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines, CXC)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Recombinant Proteins)
RN  - 11028-71-0 (Concanavalin A)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Chemokine CXCL10
MH  - Chemokines, CXC/metabolism
MH  - Cloning, Molecular
MH  - Concanavalin A/pharmacology
MH  - Female
MH  - Lymphocyte Activation
MH  - Lymphoid Tissue/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics/immunology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Receptors, Chemokine/*biosynthesis/*genetics/physiology
MH  - Recombinant Proteins/metabolism
MH  - Spleen/metabolism
MH  - T-Lymphocytes, Cytotoxic/immunology/metabolism
MH  - Transfection
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - S0006-291X(98)99404-9 [pii]
AID - 10.1006/bbrc.1998.9404 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Oct 9;251(1):41-8. doi: 10.1006/bbrc.1998.9404.