PMID- 9791008
OWN - NLM
STAT- MEDLINE
DCOM- 19981203
LR  - 20201209
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 251
IP  - 3
DP  - 1998 Oct 29
TI  - The use of the yeast two hybrid system to evaluate ErbB-3 interactions with SH2 
      domain containing proteins.
PG  - 903-6
AB  - Several mutations in the tyrosine kinase domain of ErbB-3 have been postulated to 
      render this enzyme catalytically inactive. To test which amino acid mutations in 
      ErbB-3 might be critical for kinase inactivation, we used a yeast two hybrid 
      assay of protein-protein interaction. We monitored restoration of ErbB-3 kinase 
      activity by investigating the ability of wild type or mutant ErbB-3 to associate 
      with the SH2 containing proteins Syp and Phosphatidyl-inositol-3-kinase (PI3K). 
      Our results demonstrate that changing individual amino acids to tyrosine kinase 
      consensus sequences did not increase the interaction of ErbB-3 with Syp or PI3K. 
      Mutation of the consensus Asp832 of rat ErbB-3 to Asn observed in human and 
      bovine ErbB-3 significantly increased the interaction of ErbB-3 and Syp and PI3K 
      11 or 26 fold respectively. A double mutant (Asp832Asn, Asp757 His) exhibited a 
      96 or 350 fold increase in the ability to bind PI3K and Syp.
CI  - Copyright 1998 Academic Press.
FAU - Yoo, J Y
AU  - Yoo JY
AD  - Molecular and Cellular Biology Program, University of Maryland, Baltimore, 
      Maryland, 21201, USA.
FAU - Hamburger, A W
AU  - Hamburger AW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Receptor, ErbB-3)
RN  - EC 3.1.3.48 (PTPN11 protein, human)
RN  - EC 3.1.3.48 (PTPN6 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpn11 protein, rat)
RN  - EC 3.1.3.48 (Ptpn6 protein, rat)
RN  - EC 3.1.3.48 (SH2 Domain-Containing Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Consensus Sequence
MH  - ErbB Receptors/genetics/*metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mutagenesis
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Protein Binding
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Rats
MH  - Receptor, ErbB-3
MH  - SH2 Domain-Containing Protein Tyrosine Phosphatases
MH  - Saccharomyces cerevisiae/genetics
MH  - Species Specificity
MH  - *src Homology Domains
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - S0006-291X(98)99565-1 [pii]
AID - 10.1006/bbrc.1998.9565 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Oct 29;251(3):903-6. doi: 
      10.1006/bbrc.1998.9565.