PMID- 9792292
OWN - NLM
STAT- MEDLINE
DCOM- 19981229
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 103
IP  - 1
DP  - 1998 Oct
TI  - Detection of karyotypic aberrations in acute myeloblastic leukaemia: a 
      prospective comparison between PCR/FISH and standard cytogenetics in 140 patients 
      with de novo AML.
PG  - 72-8
AB  - In 140 patients with de novo acute myeloid leukaemia (AML) standard cytogenetics 
      were compared with RT-PCR for the detection of t(8;21), t(15;17) and inv(16) and 
      fluorescence in situ hybridization (FISH) for numerical aberrations of 
      chromosomes 7, 8, X and Y. RT-PCR detected 18 cases with t(8;21), 12 with 
      t(15;17) and seven with inv(16). In two cases with t(8;21), two with t(15;17) and 
      four with inv(16) these aberrations had not been detected by standard 
      cytogenetics. There were no false negative PCR results. In 12 patients with these 
      chromosomal changes, standard cytogenetics revealed additional chromosomal 
      aberrations. In 16 patients sole numerical aberrations of the chromosomes 7, 8, X 
      or Y were found by FISH. In these patients the sensitivity of FISH and standard 
      cytogenetics was comparable. In 87 patients no aberrations could be found by PCR 
      and FISH whereas in 24 of these patients standard cytogenetics revealed an 
      abnormal karyotype. These data recommend the combination of standard cytogenetics 
      and molecular techniques to improve the sensitivity for the detection of genetic 
      lesions in AML. Once chromosomal markers have been identified by combined 
      analysis these markers could be used to monitor residual disease during/after 
      chemotherapy, by RT-PCR and/or FISH.
FAU - Krauter, J
AU  - Krauter J
AD  - Department of Haematology/Oncology, Hannover Medical School, Germany.
FAU - Peter, W
AU  - Peter W
FAU - Pascheberg, U
AU  - Pascheberg U
FAU - Heinze, B
AU  - Heinze B
FAU - Bergmann, L
AU  - Bergmann L
FAU - Hoelzer, D
AU  - Hoelzer D
FAU - Lubbert, M
AU  - Lubbert M
FAU - Schlimok, G
AU  - Schlimok G
FAU - Arnold, R
AU  - Arnold R
FAU - Kirchner, H
AU  - Kirchner H
FAU - Port, M
AU  - Port M
FAU - Ganser, A
AU  - Ganser A
FAU - Heil, G
AU  - Heil G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - 10.1046/j.1365-2141.1998.00926.x [doi]
PST - ppublish
SO  - Br J Haematol. 1998 Oct;103(1):72-8. doi: 10.1046/j.1365-2141.1998.00926.x.