PMID- 9792295
OWN - NLM
STAT- MEDLINE
DCOM- 19981229
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 103
IP  - 1
DP  - 1998 Oct
TI  - Cell lineage specific involvement in acute promyelocytic leukaemia (APL) using a 
      combination of May-Grunwald-Giemsa staining and fluorescence in situ 
      hybridization techniques for the detection of the translocation 
      t(15;17)(q22;q12).
PG  - 93-9
AB  - Acute promyelocytic leukaemia (APL) is strongly associated with the translocation 
      t(15;17) which therefore provides a reliable marker to assess the potential 
      involvement of different cell lineages. Six cases with morphologically, 
      cytogenetically and molecularly proven APL were analysed at diagnosis or relapse 
      by combining fluorescence in situ hybridization (FISH) with standard 
      May-Grunwald-Giemsa (MGG) staining at the single cell level on bone marrow and 
      blood smears. With the FICTION technique, combining immunophenotyping with FISH, 
      haemopoietic precursor cells were identified using monoclonal antibodies against 
      CD34, B- and T-lymphocytes could be identified with CD19 and CD3. In addition, 
      HLA-DR-positive cells were studied for the presence of t(15;17). Morphologically 
      identified myeloblasts were relocated on the smear after FISH and were found to 
      be PML/RARA positive in 91%, abnormal promyelocytes in 97%. In contrast, a 
      positive signal was obtained in only 18% of PMN. Erythroblasts, lymphocytes and 
      plasma cells did not show a PML/RARA rearrangement. Accordingly, all cells 
      expressing CD3 or CD19 were PML/RARA negative. CD34 positive precursor cells 
      identified by FICTION were PML/RARA positive in 97%. HLA-DR-positive cells 
      contained a PML/RARA rearrangement in 24% of cells in one case and were negative 
      in the two other cases investigated. These data indicate that APL appears to 
      originate from a level of haemopoietic precursor cells but is restricted to the 
      myeloid lineage. The low proportion of PML/RARA-positive PMN points to the 
      impairment of blast cell differentiation beyond the promyelocyte stage but also 
      emphasizes the existence of normal residual haemopoietic stem cells from which 
      PML/RARA-negative PMN must be derived.
FAU - Haferlach, T
AU  - Haferlach T
AD  - Department of Haematology and Oncology, University of Gottingen, Germany.
FAU - Loffler, H
AU  - Loffler H
FAU - Nickenig, C
AU  - Nickenig C
FAU - Ramm-Petersen, L
AU  - Ramm-Petersen L
FAU - Meeder, M
AU  - Meeder M
FAU - Schoch, R
AU  - Schoch R
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Schnittger, S
AU  - Schnittger S
FAU - Schoch, C
AU  - Schoch C
FAU - Hiddemann, W
AU  - Hiddemann W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Azure Stains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Azure Stains
MH  - Cell Lineage
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Female
MH  - Humans
MH  - Immunophenotyping/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - 10.1046/j.1365-2141.1998.00959.x [doi]
PST - ppublish
SO  - Br J Haematol. 1998 Oct;103(1):93-9. doi: 10.1046/j.1365-2141.1998.00959.x.