PMID- 9792466
OWN - NLM
STAT- MEDLINE
DCOM- 19990108
LR  - 20061115
IS  - 0947-7349 (Print)
IS  - 0947-7349 (Linking)
VI  - 106
IP  - 4
DP  - 1998
TI  - Regulation of interleukin-6 expression in human osteoblastic cells in vitro.
PG  - 324-33
AB  - Interleukin-6 (IL-6) is a potent stimulator of bone resorption which has been 
      demonstrated in a variety of in vivo and in vitro models. We investigated the 
      regulation of IL-6 secretion in primary human osteoblastic cells (HOC) in vitro 
      by cytokines known to play an important role in coupling bone formation to bone 
      resorption. HOC were isolated from healthy adults who underwent selective 
      orthopedic surgery and treated with cytokines released in the bone 
      microenvironment during coupling i.e Interleukin-1beta (IL-1beta), Tumor Necrosis 
      Factor alpha (TNFalpha), Transforming Growth Factor beta1 and 2 (TGFbeta 1 and 2) 
      and Endothelin-1 (ET-1). Furthermore, we determined whether systemically-acting 
      steroid hormones of gonadal and adrenal origin as well as glucocorticoids affect 
      the local regulation of IL-6 secretion in primary HOC. To examine the effects of 
      different steroid hormones on IL-6 production, HOC were exposed to estradiol 
      (E2), dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA) and dexamethasone 
      (Dexa) with and without a subsequent treatment of the HOC populations with 
      cytokines. We observed that (1) IL-1beta and TNFalpha induced IL-6 in a dose and 
      time-dependent fashion, (2) TGFbeta 1 and 2 enhanced basal and IL-1beta and 
      TNFalpha induced IL-6 expression, (3) ET-1 elicited a dose-dependent stimulatory 
      effect on IL-6 expression. (4) E2, DHT and DHEA alone and in combination with 
      IL-1beta and TNFalpha elicited no reproducible dose-dependent effect on IL-6 
      production, whereas Dexa inhibited basal and IL-1beta and TNFalpha induced IL-6 
      expression dose dependently. In conclusion, IL-1beta, TNFalpha, TGFbeta 1 and 2 
      and ET-1 may participate in the regulation of bone resorption by stimulating IL-6 
      expression in HOC. Dexa inhibits the constitutive and cytokine stimulated IL-6 
      expression, whereas there is no in vitro evidence that sex steroids exert a major 
      inhibitory effect on the osteoblastic secretion of IL-6 as demonstrated in a 
      primary human bone cell model.
FAU - Hierl, T
AU  - Hierl T
AD  - Ruprecht-Karls-University of Heidelberg, Department of Medicine, Germany.
FAU - Borcsok, I
AU  - Borcsok I
FAU - Sommer, U
AU  - Sommer U
FAU - Ziegler, R
AU  - Ziegler R
FAU - Kasperk, C
AU  - Kasperk C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Exp Clin Endocrinol Diabetes
JT  - Experimental and clinical endocrinology & diabetes : official journal, German 
      Society of Endocrinology [and] German Diabetes Association
JID - 9505926
RN  - 0 (Cytokines)
RN  - 0 (Endothelins)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Cytokines/pharmacology
MH  - Endothelins/pharmacology
MH  - Female
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/*biosynthesis
MH  - Lymphotoxin-alpha/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Osteoblasts/drug effects/*metabolism
MH  - RNA, Messenger/biosynthesis/isolation & purification
MH  - Steroids/pharmacology
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1998/10/29 00:00
MHDA- 1998/10/29 00:01
CRDT- 1998/10/29 00:00
PHST- 1998/10/29 00:00 [pubmed]
PHST- 1998/10/29 00:01 [medline]
PHST- 1998/10/29 00:00 [entrez]
AID - 10.1055/s-0029-1211993 [doi]
PST - ppublish
SO  - Exp Clin Endocrinol Diabetes. 1998;106(4):324-33. doi: 10.1055/s-0029-1211993.