PMID- 9792830
OWN - NLM
STAT- MEDLINE
DCOM- 19981123
LR  - 20071114
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 251
IP  - 2
DP  - 1998 Oct 20
TI  - Markedly increased constitutive CYP1A1 mRNA levels in the fertilized ovum of the 
      mouse.
PG  - 657-61
AB  - Using a highly sensitive RT-PCR technique that measures mRNA (cDNA)-to-DNA 
      ratios, we are able to detect constitutive CYP1A1 mRNA in adult mouse liver as 
      well as in the oocyte. Twelve hours after fertilization of the ovum, there is a 
      more than 100-fold increase in constitutive CYP1A1 mRNA levels; this dramatic 
      increase completely disappears by the 2-cell stage at gestational day 1.5 
      (GD1.5), as well as in the blastocyst at GD3.5. The CYP1A1 enzyme has been shown 
      to remove an endogenous ligand for the Ah receptor (AHR), and the AHR is known to 
      play a role in cell cycle regulation and apoptosis. We therefore postulate that 
      this striking abundance of constitutive CYP1A1 mRNA in the fertilized ovum at 
      GD0.5 might be important for maintaining sufficient amounts of the CYP1A1 enzyme 
      during the transition from maternal to zygotic control (GD0.5 to GD1.5 embryo); 
      availability of catalytically active CYP1A1 would ensure that any (exogenous or 
      endogenous) AHR ligand be rapidly degraded, so that all undesirable AHR-mediated 
      gene transcription would be prevented during these very critical moments of early 
      mammalian embryogenesis.
CI  - Copyright 1998 Academic Press.
FAU - Dey, A
AU  - Dey A
AD  - Center for Environmental Genetics and the Department of Environmental Health, 
      University of Cincinnati Medical Center, Cincinnati, Ohio, 45267-0056, USA.
FAU - Nebert, D W
AU  - Nebert DW
LA  - eng
GR  - P30 ES06096/ES/NIEHS NIH HHS/United States
GR  - R01 ES08147/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blastocyst/enzymology
MH  - Cell Cycle
MH  - Cytochrome P-450 CYP1A1/*genetics
MH  - Embryonic and Fetal Development
MH  - Exons
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - Ligands
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oocytes/enzymology
MH  - Ovum/enzymology
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Receptors, Aryl Hydrocarbon/metabolism
MH  - Superovulation
MH  - *Transcription, Genetic
MH  - Zygote/*enzymology
EDAT- 1998/10/30 00:00
MHDA- 1998/10/30 00:01
CRDT- 1998/10/30 00:00
PHST- 1998/10/30 00:00 [pubmed]
PHST- 1998/10/30 00:01 [medline]
PHST- 1998/10/30 00:00 [entrez]
AID - S0006-291X(98)99519-5 [pii]
AID - 10.1006/bbrc.1998.9519 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Oct 20;251(2):657-61. doi: 
      10.1006/bbrc.1998.9519.