PMID- 9792940
OWN - NLM
STAT- MEDLINE
DCOM- 19981202
LR  - 20171208
IS  - 0001-5792 (Print)
IS  - 0001-5792 (Linking)
VI  - 100
IP  - 2
DP  - 1998
TI  - Fluorescence in situ hybridization and surface markers of fine needle aspiration 
      specimen confirm extramedullary myeloblastoma in a patient with chronic myeloid 
      leukemia.
PG  - 91-3
AB  - We report on a patient with chronic myeloid leukemia (CML) with a rapidly growing 
      left cervical tumor 5 months after the initial diagnosis of CML. This tumor was 
      diagnosed as a very early manifestation of extramedullary myeloblastoma by a 
      minimally invasive method. A fine needle aspirate (26-gauge needle) was obtained 
      from the tumor. Morphological and cytochemical analysis of the aspirate revealed 
      19% undifferentiated blasts. The immunophenotype was suggestive of a myeloid 
      differentiation of the blasts (CD33+). The CML origin of the blasts was confirmed 
      by the detection of the bcr-abl gene rearrangement in the blasts by two-color 
      fluorescence in situ hybridization (FISH). We conclude that fine needle 
      aspiration in combination with immunophenotyping and FISH analysis of the 
      aspirate is a minimally invasive and rapid diagnostic tool to confirm 
      extramedullary manifestation in CML. To our knowledge, this is the first case of 
      extramedullary myeloblastoma confirmed by this combined technique.
FAU - Karthaus, M
AU  - Karthaus M
AD  - Department of Haematology and Oncology, Hannover Medical School, Germany.
FAU - Meran, J G
AU  - Meran JG
FAU - Wilkens, L
AU  - Wilkens L
FAU - Soudah, B
AU  - Soudah B
FAU - Diedrich, H
AU  - Diedrich H
FAU - Krauter, J
AU  - Krauter J
FAU - Ganser, A
AU  - Ganser A
FAU - Heil, G
AU  - Heil G
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - Switzerland
TA  - Acta Haematol
JT  - Acta haematologica
JID - 0141053
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Antigens, Surface/*analysis
MH  - Biomarkers, Tumor/*analysis
MH  - Biopsy, Needle
MH  - Blast Crisis/metabolism/pathology
MH  - Female
MH  - Genes, abl
MH  - *Hematopoiesis, Extramedullary
MH  - Hematopoietic Stem Cells/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism/*pathology
MH  - Uterine Cervical Neoplasms/chemistry/pathology
EDAT- 1998/10/30 03:02
MHDA- 2000/08/16 11:00
CRDT- 1998/10/30 03:02
PHST- 1998/10/30 03:02 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/10/30 03:02 [entrez]
AID - 40873 [pii]
AID - 10.1159/000040873 [doi]
PST - ppublish
SO  - Acta Haematol. 1998;100(2):91-3. doi: 10.1159/000040873.