PMID- 9794406
OWN - NLM
STAT- MEDLINE
DCOM- 19981118
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 161
IP  - 9
DP  - 1998 Nov 1
TI  - NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated 
      factors in its signaling and identification of functional region of CD27.
PG  - 4753-9
AB  - CD27 belongs to TNF receptor family, and it is unique in this family for its 
      disulfide-linked homodimerization of 55-kDa monomers. In the present study we 
      demonstrate that overexpression of CD27 in 293 cells induces a low level of 
      NF-kappaB activation, and the ligation of the receptor by its corresponding 
      ligand (CD70) augments this signal dramatically. Either TNF receptor-associated 
      factor-2 (TRAF2) or TRAF3 binds to the CD27 molecule from the 
      coimmunoprecipitation experiment. This NF-kappaB activation signal is inhibited 
      by dominant negative TRAF2 or intact TRAF3, indicating that TRAF2 and TRAF3 works 
      as a mediator and an inhibitor, respectively. The activated NF-kappaB complex 
      contains at least two components, p50 and p65, but not p52. All these phenomena 
      have also been observed in the TNF receptor type II, CD30 and CD40 signaling 
      system, indicating that this receptor family uses the common or similar molecules 
      for this signal. Finally, we identified the 13-amino acid alignment in the 
      cytoplasmic region of the CD27 molecule (residues 238-250 amino acids), which is 
      critical for the NF-kappaB activation signal and also for its association with 
      TRAFs. This amino acid alignment contains the EEEG sequence, which is essential 
      for interaction of CD30 or CD40 with TRAFs (TRAF1 and TRAF2, but not TRAF3), and 
      also contains the PIQED sequence, which is similar to PXQXT that is known to be 
      necessary for interaction of TNF receptor II and CD30 with TRAFs (TRAF1, 2, and 
      3).
FAU - Yamamoto, H
AU  - Yamamoto H
AD  - Department of Medicine III, Osaka University Medical School, Japan.
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Minamoto, S
AU  - Minamoto S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (CD27 Ligand)
RN  - 0 (CD70 protein, human)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (TNF Receptor-Associated Factor 1)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (TNF Receptor-Associated Factor 3)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Antigens, CD
MH  - CD27 Ligand
MH  - Cell Line
MH  - Dimerization
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Macromolecular Substances
MH  - Membrane Proteins/physiology
MH  - NF-kappa B/*physiology
MH  - Proteins/*physiology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/*physiology
MH  - TNF Receptor-Associated Factor 1
MH  - TNF Receptor-Associated Factor 2
MH  - TNF Receptor-Associated Factor 3
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/chemistry/*physiology
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 1998/10/30 00:00
MHDA- 1998/10/30 00:01
CRDT- 1998/10/30 00:00
PHST- 1998/10/30 00:00 [pubmed]
PHST- 1998/10/30 00:01 [medline]
PHST- 1998/10/30 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Nov 1;161(9):4753-9.