PMID- 9794468
OWN - NLM
STAT- MEDLINE
DCOM- 19981106
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 139
IP  - 11
DP  - 1998 Nov
TI  - Production and secretion of adrenomedullin in cultured rat cardiac myocytes and 
      nonmyocytes: stimulation by interleukin-1beta and tumor necrosis factor-alpha.
PG  - 4576-80
AB  - The present study investigated the secretion level and gene expression of 
      adrenomedullin (AM), a novel vasorelaxant peptide, in cultured neonatal rat 
      cardiac myocytes and nonmyocytes, and the effects of interleukin-1beta (IL-1beta) 
      and tumor necrosis factor-alpha (TNF alpha) on its production and secretion in 
      these cells. Under serum-free conditions, both myocytes and nonmyocytes secreted 
      immunoreactive (ir-) AM into the culture medium in a time-dependent manner. The 
      secretion rates of ir-AM from myocytes and nonmyocytes per 10(5) cells were 
      almost equivalent. The expression of AM messenger RNA was also observed in 
      cultured myocytes and nonmyocytes. The peptide secretion and messenger RNA level 
      of AM in cardiac myocytes were increased after stimulation with IL-1beta. In 
      nonmyocytes, IL-1beta and TNF alpha remarkably augmented both the release of 
      ir-AM into the medium and AM gene expression after 24 and 48 h of incubation. 
      These observations indicate that cardiac ventricular cells (i.e. myocytes and 
      nonmyocytes) actively produce AM and also suggest that cytokines such as IL-1beta 
      and TNF alpha regulate the gene expression and secretion of this peptide in the 
      ventricles. On the basis of these results and the findings that IL-1beta and TNF 
      alpha are involved in heart failure and cardiac hypertrophy, AM may play a role 
      as an autocrine/paracrine modulator in some cardiac disorders.
FAU - Horio, T
AU  - Horio T
AD  - Department of Medicine, National Cardiovascular Center, Suita, Osaka, Japan.
FAU - Nishikimi, T
AU  - Nishikimi T
FAU - Yoshihara, F
AU  - Yoshihara F
FAU - Nagaya, N
AU  - Nagaya N
FAU - Matsuo, H
AU  - Matsuo H
FAU - Takishita, S
AU  - Takishita S
FAU - Kangawa, K
AU  - Kangawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Culture Media)
RN  - 0 (Endothelin-1)
RN  - 0 (Interleukin-1)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11128-99-7 (Angiotensin II)
RN  - 148498-78-6 (Adrenomedullin)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 85637-73-6 (Atrial Natriuretic Factor)
SB  - IM
MH  - Adrenomedullin
MH  - Angiotensin II/pharmacology
MH  - Animals
MH  - Atrial Natriuretic Factor/biosynthesis
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Culture Media
MH  - Endothelin-1/pharmacology
MH  - Heart/drug effects
MH  - Interleukin-1/*pharmacology
MH  - Myocardium/cytology/*metabolism
MH  - Peptides/*metabolism
MH  - Phenylephrine/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1998/10/30 00:00
MHDA- 1998/10/30 00:01
CRDT- 1998/10/30 00:00
PHST- 1998/10/30 00:00 [pubmed]
PHST- 1998/10/30 00:01 [medline]
PHST- 1998/10/30 00:00 [entrez]
AID - 10.1210/endo.139.11.6306 [doi]
PST - ppublish
SO  - Endocrinology. 1998 Nov;139(11):4576-80. doi: 10.1210/endo.139.11.6306.