PMID- 9794812
OWN - NLM
STAT- MEDLINE
DCOM- 19981228
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 335 ( Pt 3)
IP  - Pt 3
DP  - 1998 Nov 1
TI  - Demonstration of a RNA-dependent nuclear interaction between the promyelocytic 
      leukaemia protein and glyceraldehyde-3-phosphate dehydrogenase.
PG  - 691-6
AB  - The promyelocytic leukaemia (protein) (PML) localizes to multiprotein complexes 
      known as PML nuclear bodies. We found that glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) co-immunoprecipitates with PML and co-localizes with PML in 
      nuclear bodies. RNase treatment disrupts the ability of PML and GAPDH to both 
      co-localize and co-immunoprecipitate, indicating that the association between PML 
      and GAPDH depends on the presence of RNA. Disruption of PML bodies contributes 
      towards reduced apoptosis in acute promyelocytic leukaemia and GAPDH induces 
      apoptotic neuronal death. The GAPDH-PML interaction may be involved in the 
      regulation of apoptosis.
FAU - Carlile, G W
AU  - Carlile GW
AD  - Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova 
      Scotia, Canada.
FAU - Tatton, W G
AU  - Tatton WG
FAU - Borden, K L
AU  - Borden KL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Pml protein, mouse)
RN  - 0 (Promyelocytic Leukemia Protein)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 143220-95-5 (PML protein, human)
RN  - 63231-63-0 (RNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fibroblasts
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/isolation & purification/*metabolism
MH  - Humans
MH  - Mice
MH  - Neoplasm Proteins/isolation & purification/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Promyelocytic Leukemia Protein
MH  - RNA/*metabolism
MH  - Ribonucleases
MH  - Transcription Factors/isolation & purification/*metabolism
MH  - Tumor Suppressor Proteins
PMC - PMC1219833
EDAT- 1998/10/31 00:00
MHDA- 1998/10/31 00:01
PMCR- 1999/05/01
CRDT- 1998/10/31 00:00
PHST- 1998/10/31 00:00 [pubmed]
PHST- 1998/10/31 00:01 [medline]
PHST- 1998/10/31 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 10.1042/bj3350691 [doi]
PST - ppublish
SO  - Biochem J. 1998 Nov 1;335 ( Pt 3)(Pt 3):691-6. doi: 10.1042/bj3350691.