PMID- 9796097
OWN - NLM
STAT- MEDLINE
DCOM- 19981125
LR  - 20190915
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 41
IP  - 4
DP  - 1998 Aug
TI  - Cytological and molecular characterization of repetitive DNA sequences of Solanum 
      brevidens and Solanum tuberosum.
PG  - 487-94
AB  - The chromosomal distribution, copy numbers, and nucleotide sequences were 
      determined for four repetitive DNA clones, pSB1 and pSB7 of Solanum brevidens and 
      pST3 and pST10 of Solanum tuberosum. Using fluorescence in situ hybridization 
      (FISH), pSB1 and pSB7 were localized near the telomeres and in some centromeric 
      and interstitial sites of S. brevidens chromosomes, but not in S. tuberosum 
      chromosomes, after high stringency washes. The clone pST3 showed signals in the 
      telomeric areas of a few chromosomes in S. tuberosum, but signals were not 
      detected in S. brevidens. All three repeated sequences (pSB1, pSB7, and pST3) 
      were detected in chromosomal areas that are typically known to contain tandemly 
      repeated sequences. The S. tuberosum clone pST10 did not show signals in either 
      species even at low stringency conditions. The estimated copy numbers of the four 
      clones were 1500, 6750, 300, and 400 for pSB1, pSB7, pST3, and pST10, 
      respectively, in the corresponding haploid genomes (S. brevidens and S. 
      tuberosum). The inserts of the four clones pSB1, pSB7, pST3, and pST10 were 322, 
      167, 845 and 121 bp, respectively. After sequencing, no significant sequence 
      homologies were found among the four clones. A homology search in sequence data 
      bases showed that pSB7 has variable homology (78-100%) with another repetitive 
      sequence of S. brevidens Sb4/2 depending on its subrepeat. It also showed some 
      homology with one repeat of tomato (pLEG15) and one repeat of Solanum 
      circaeifolium (pSC15).
FAU - Rokka, V M
AU  - Rokka VM
AD  - Agricultural Research Centre of Finland, Institute of Crop and Soil Science, 
      Jokioinen, Finland. veli-matti.rokka@mtt.fi
FAU - Clark, M S
AU  - Clark MS
FAU - Knudson, D L
AU  - Knudson DL
FAU - Pehu, E
AU  - Pehu E
FAU - Lapitan, N L
AU  - Lapitan NL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes/ultrastructure
MH  - DNA, Plant/chemistry/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Repetitive Sequences, Nucleic Acid
MH  - Solanaceae/*genetics
MH  - Solanum tuberosum/genetics
EDAT- 1998/10/31 00:00
MHDA- 1998/10/31 00:01
CRDT- 1998/10/31 00:00
PHST- 1998/10/31 00:00 [pubmed]
PHST- 1998/10/31 00:01 [medline]
PHST- 1998/10/31 00:00 [entrez]
AID - 10.1139/g98-047 [doi]
PST - ppublish
SO  - Genome. 1998 Aug;41(4):487-94. doi: 10.1139/g98-047.