PMID- 9797106
OWN - NLM
STAT- MEDLINE
DCOM- 19981112
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 70
IP  - 4
DP  - 1998 Oct
TI  - Potential use of repeated fluorescence in situ hybridization in the same human 
      blastomeres for preimplantation genetic diagnosis.
PG  - 729-33
AB  - OBJECTIVE: To assess the feasibility of repeated fluorescence in situ 
      hybridization (FISH) procedures in the same nucleus of a human blastomere. 
      DESIGN: Three consecutive FISH procedures were performed in the same human 
      blastomere by using direct label fluorescence CEP and WCP probes (Vysis). 
      SETTING: Hospital-based private IVF program. PATIENT(S): Twenty-eight infertile 
      couples who underwent conventional IVF in our center. INTERVENTION(S): Embryos 
      from oocytes with three pronuclei after in vitro insemination were used in this 
      study. MAIN OUTCOME MEASURE(S): The rates of nuclear loss, present signals, and 
      absent signal were examined. RESULT(S): In group 1, the rates of presence of 
      signals were 94% after the first FISH, 92% after the second FISH, and 88% after 
      the third FISH. In group 2, the rates of presence of signals were 96% after the 
      first FISH, 93% after the second FISH, and 87% after the third FISH. There was no 
      statistically significant difference in the rates of nuclear loss, present 
      signals, and absent signal between three consecutive FISH procedures and between 
      CEP and WCP probes. CONCLUSION(S): Six or more chromosomes of a single blastomere 
      may be examined with use of this repeated FISH procedure, which may be important 
      for preimplantation genetic diagnosis.
FAU - Liu, J
AU  - Liu J
AD  - The Greater Baltimore Medical Center Fertility Center, Maryland 21204, USA. 
      jliu@gbmc.org
FAU - Tsai, Y L
AU  - Tsai YL
FAU - Zheng, X Z
AU  - Zheng XZ
FAU - Baramki, T A
AU  - Baramki TA
FAU - Yazigi, R A
AU  - Yazigi RA
FAU - Katz, E
AU  - Katz E
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - *Blastomeres
MH  - *Embryonic Development
MH  - Female
MH  - *Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
EDAT- 1998/10/31 00:00
MHDA- 1998/10/31 00:01
CRDT- 1998/10/31 00:00
PHST- 1998/10/31 00:00 [pubmed]
PHST- 1998/10/31 00:01 [medline]
PHST- 1998/10/31 00:00 [entrez]
AID - S0015-0282(98)00244-1 [pii]
AID - 10.1016/s0015-0282(98)00244-1 [doi]
PST - ppublish
SO  - Fertil Steril. 1998 Oct;70(4):729-33. doi: 10.1016/s0015-0282(98)00244-1.