PMID- 9797297
OWN - NLM
STAT- MEDLINE
DCOM- 19990114
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 64
IP  - 11
DP  - 1998 Nov
TI  - Molecular detection, isolation, and physiological characterization of 
      functionally dominant phenol-degrading bacteria in activated sludge.
PG  - 4396-402
AB  - DNA was isolated from phenol-digesting activated sludge, and partial fragments of 
      the 16S ribosomal DNA (rDNA) and the gene encoding the largest subunit of 
      multicomponent phenol hydroxylase (LmPH) were amplified by PCR. An analysis of 
      the amplified fragments by temperature gradient gel electrophoresis (TGGE) 
      demonstrated that two major 16S rDNA bands (bands R2 and R3) and two major LmPH 
      gene bands (bands P2 and P3) appeared after the activated sludge became 
      acclimated to phenol. The nucleotide sequences of these major bands were 
      determined. In parallel, bacteria were isolated from the activated sludge by 
      direct plating or by plating after enrichment either in batch cultures or in a 
      chemostat culture. The bacteria isolated were classified into 27 distinct groups 
      by a repetitive extragenic palindromic sequence PCR analysis. The partial 
      nucleotide sequences of 16S rDNAs and LmPH genes of members of these 27 groups 
      were then determined. A comparison of these nucleotide sequences with the 
      sequences of the major TGGE bands indicated that the major bacterial populations, 
      R2 and R3, possessed major LmPH genes P2 and P3, respectively. The dominant 
      populations could be isolated either by direct plating or by chemostat culture 
      enrichment but not by batch culture enrichment. One of the dominant strains (R3) 
      which contained a novel type of LmPH (P3), was closely related to Valivorax 
      paradoxus, and the result of a kinetic analysis of its phenol-oxygenating 
      activity suggested that this strain was the principal phenol digester in the 
      activated sludge.
FAU - Watanabe, K
AU  - Watanabe K
AD  - Marine Biotechnology Institute, Kamaishi Laboratories, Heita, Kamaishi City, 
      Iwate, Japan. kazwata@kamaishi.mbio.co.jp
FAU - Teramoto, M
AU  - Teramoto M
FAU - Futamata, H
AU  - Futamata H
FAU - Harayama, S
AU  - Harayama S
LA  - eng
SI  - GENBANK/AB011551
SI  - GENBANK/AB011552
SI  - GENBANK/AB011553
SI  - GENBANK/AB011554
SI  - GENBANK/AB011555
SI  - GENBANK/AB011556
SI  - GENBANK/AB011557
SI  - GENBANK/AB011558
SI  - GENBANK/AB011559
SI  - GENBANK/AB011560
SI  - GENBANK/AB011561
SI  - GENBANK/AB011562
SI  - GENBANK/AB011563
SI  - GENBANK/AB011564
SI  - GENBANK/AB011565
SI  - GENBANK/AB011566
SI  - GENBANK/AB011567
SI  - GENBANK/AB011568
SI  - GENBANK/AB011569
SI  - GENBANK/AB011570
SI  - GENBANK/AB011571
SI  - GENBANK/AB011572
SI  - GENBANK/AB011573
SI  - GENBANK/AB011574
SI  - GENBANK/AB011575
SI  - GENBANK/AB011576
SI  - GENBANK/AB011577
SI  - GENBANK/AB011578
SI  - GENBANK/AB011579
SI  - GENBANK/AB011580
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Phenols)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.13.7 (phenol 2-monooxygenase)
SB  - IM
MH  - Acinetobacter/genetics/isolation & purification
MH  - Bacteria/genetics/*isolation & purification/metabolism
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - DNA Primers
MH  - DNA, Ribosomal/genetics
MH  - Macromolecular Substances
MH  - Mixed Function Oxygenases/*genetics
MH  - Molecular Sequence Data
MH  - Nocardiaceae/genetics/isolation & purification
MH  - Phenols/*metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Pseudomonas/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/*genetics/isolation & purification
MH  - Rhodobacter/genetics/isolation & purification
MH  - Sewage/*microbiology
MH  - Xanthomonas/genetics/isolation & purification
PMC - PMC106659
EDAT- 1998/10/31 00:00
MHDA- 1998/10/31 00:01
PMCR- 1998/11/01
CRDT- 1998/10/31 00:00
PHST- 1998/10/31 00:00 [pubmed]
PHST- 1998/10/31 00:01 [medline]
PHST- 1998/10/31 00:00 [entrez]
PHST- 1998/11/01 00:00 [pmc-release]
AID - 0394 [pii]
AID - 10.1128/AEM.64.11.4396-4402.1998 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1998 Nov;64(11):4396-402. doi: 
      10.1128/AEM.64.11.4396-4402.1998.