PMID- 9797772
OWN - NLM
STAT- MEDLINE
DCOM- 19981104
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 106
IP  - 2
DP  - 1998 Oct 15
TI  - Clear-cell and papillary carcinoma of the kidney: an analysis of chromosome 3, 7, 
      and 17 abnormalities by microsatellite amplification, cytogenetics, and 
      fluorescence in situ hybridization.
PG  - 93-104
AB  - Clear-cell and papillary renal cell carcinomas (RCCs) have specific genetic 
      changes that allow them to be classified on the basis of histopathology and on 
      the basis of cytogenetic and molecular genetic findings. Clear-cell carcinomas 
      are characterized by a deletion of gene sequences on the short arm of chromosome 
      3 (3p). Papillary RCCs do not have 3p deletions but have an increase in 
      chromosomal number that usually includes trisomies of chromosomes 7 and 17. This 
      study was undertaken to determine whether PCR-amplified DNA microsatellites can 
      be used to detect numerical abnormalities of chromosomes 7 and 17 and whether the 
      numerical abnormalities and 3p deletions that are detected by microsatellite 
      analysis can be correlated with histopathologic tumor types. A series of 
      histologically unambiguous RCCs consisting of three papillary and ten clear-cell 
      RCCs were studied by cytogenetics and by fluorescence in situ hybridization 
      (FISH) with chromosome 7 and 17 centromeric probes. Microsatellites on the long 
      and short arms of chromosomes 3, 7, and 17 were amplified in paired normal tissue 
      and tumor samples, and the reaction products were analyzed for differences 
      between the normal and the tumor allele ratios. Clear-cell carcinomas showed loss 
      of heterozygosity (LOH) of 3p but not 3q alleles in eight of ten cases. LOH of 3p 
      and 3q was seen in one case of papillary RCC that cytogenetically had two normal 
      chromosomes 3. This indicated a nondisjunction duplication that could be confused 
      with monosomy 3 if only microsatellite studies were performed. Differences in 
      microsatellite allele ratios between normal tissue and tumor correlated with the 
      presence of trisomy 7 that was identified in clear-cell and papillary RCCs by 
      cytogenetics and by FISH. Microsatellite analysis did not detect numerical 
      chromosome 17 abnormalities in the papillary RCCs but did show an abnormality in 
      one clear-cell carcinoma that was markedly aneusomic for chromosomes 7 and 17 by 
      FISH. In this collection of cases, microsatellite amplification genetically 
      distinguished only clear-cell RCCs showing 3p but not 3q LOH as a separate class 
      of tumors. The method detected abnormalities in chromosome number that were found 
      in both clear-cell and papillary RCCs.
FAU - Hughson, M D
AU  - Hughson MD
AD  - Department of Pathology, University of Mississippi Medical Center, Jackson 
      39216-4505, USA.
FAU - Dickman, K
AU  - Dickman K
FAU - Bigler, S A
AU  - Bigler SA
FAU - Meloni, A M
AU  - Meloni AM
FAU - Sandberg, A A
AU  - Sandberg AA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adenocarcinoma, Clear Cell/*genetics
MH  - Carcinoma, Papillary/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 3
MH  - Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Kidney Neoplasms/*genetics
MH  - Microsatellite Repeats
EDAT- 1998/11/03 00:00
MHDA- 1998/11/03 00:01
CRDT- 1998/11/03 00:00
PHST- 1998/11/03 00:00 [pubmed]
PHST- 1998/11/03 00:01 [medline]
PHST- 1998/11/03 00:00 [entrez]
AID - S0165460898000685 [pii]
AID - 10.1016/s0165-4608(98)00068-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Oct 15;106(2):93-104. doi: 
      10.1016/s0165-4608(98)00068-5.