PMID- 9797777
OWN - NLM
STAT- MEDLINE
DCOM- 19981104
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 106
IP  - 2
DP  - 1998 Oct 15
TI  - Fluorescence in situ hybridization studies of interphase nuclei for assessing 
      response to therapy in patients with chronic myeloid leukemia.
PG  - 128-34
AB  - We used two-color fluorescence in situ hybridization (FISH) to detect BCR/ABL 
      fusion in interphase nuclei in bone marrow of 17 patients with chronic myeloid 
      leukemia (CML) before and in the course of interferon therapy. The results of 
      FISH were compared with the data of conventional cytogenetic investigation (G- or 
      Q-banding) of the same specimens. Changes in percentage of Ph-positive nuclei 
      correlated with variations in percentage of Ph-positive metaphases. An overall 
      difference in the classification of patients by conventional cytogenetics and 
      FISH based on the percentage of Ph-positive cells was not observed. This FISH 
      method is reproducible, relatively easy to perform, and reliable for monitoring 
      patients with CML.
FAU - Kobzev, Y
AU  - Kobzev Y
AD  - Karyology Laboratory, National Research Center for Hematology, Moscow, Russia.
FAU - Domracheva, E
AU  - Domracheva E
FAU - Zakharova, A
AU  - Zakharova A
FAU - Khoroshko, N
AU  - Khoroshko N
FAU - Turkina, A
AU  - Turkina A
FAU - Dewald, G
AU  - Dewald G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 9008-11-1 (Interferons)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Bone Marrow/chemistry
MH  - Evaluation Studies as Topic
MH  - Fusion Proteins, bcr-abl/*analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interferons/therapeutic use
MH  - *Interphase
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/therapy
MH  - Philadelphia Chromosome
MH  - Reproducibility of Results
MH  - Treatment Outcome
EDAT- 1998/11/03 00:00
MHDA- 1998/11/03 00:01
CRDT- 1998/11/03 00:00
PHST- 1998/11/03 00:00 [pubmed]
PHST- 1998/11/03 00:01 [medline]
PHST- 1998/11/03 00:00 [entrez]
AID - S0165460898000521 [pii]
AID - 10.1016/s0165-4608(98)00052-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Oct 15;106(2):128-34. doi: 
      10.1016/s0165-4608(98)00052-1.