PMID- 9797783
OWN - NLM
STAT- MEDLINE
DCOM- 19981104
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 106
IP  - 2
DP  - 1998 Oct 15
TI  - Detection of chromosomal aberrations in tumor cells and tumor infiltrating 
      lymphocytes by molecular cytogenetics in patients with gynecological cancer.
PG  - 159-65
AB  - Conventional cytogenetic studies of tumor cells from patients with breast or 
      ovarian cancer have shown multiple chromosomal abnormalities including 
      chromosomes 7, 12, and 17. This study was designed to analyze the cytogenetic 
      features of tumor cells and tumor infiltrating lymphocytes (TILs) by using a 
      combination of magnetic activated cell sorting (MACS) and fluorescence in situ 
      hybridization (FISH). Tumor cell, peripheral blood (PB), and TIL samples from 37 
      patients (20 ovarian tumors, 13 breast cancers, 3 uterine sarcoma, 1 carcinoma of 
      the filamentary tube) were analyzed for the presence of numerical aberrations of 
      chromosomes 7, 12, and 17. All of the tumor cells showed a high frequency of 
      numerical aberrations of chromosomes 7, 12, and 17, especially trisomies or 
      tetrasomies. There was no statistically significant difference in the incidence 
      of chromosomal abnormalities in tumor tissue and effusions, or between primary 
      and relapsed disease in patients with breast or ovarian tumors. However, tumor 
      cells from patients with solid metastatic disease had significantly higher 
      numbers of aberrations of chromosome 7 in the primary tumor than in tumors from 
      patients without metastases (P = 0.049), suggesting that chromosome 7 is 
      frequently involved in the progression of disease. Monosomies and trisomies of 
      chromosomes 7 and 12 also occurred at a low percentage of TILs without any 
      statistically significant difference between primary and relapsed tumors. The 
      presence of these aneuploidies might be responsible for treatment failures in the 
      immunotherapy of gynecological cancer.
FAU - Engel, H
AU  - Engel H
AD  - Department of Gynecology and Obstetrics, University of Cologne, Germany.
FAU - Friedrich, J
AU  - Friedrich J
FAU - Kleespies, C
AU  - Kleespies C
FAU - Kurbacher, C M
AU  - Kurbacher CM
FAU - Schondorf, T
AU  - Schondorf T
FAU - Grecu, O
AU  - Grecu O
FAU - Kolhagen, H
AU  - Kolhagen H
FAU - Mallmann, P
AU  - Mallmann P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/genetics
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Genital Neoplasms, Female/*genetics
MH  - Humans
MH  - Lymphocytes, Tumor-Infiltrating/*ultrastructure
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Ovarian Neoplasms/genetics
MH  - Uterine Neoplasms/genetics
EDAT- 1998/11/03 00:00
MHDA- 1998/11/03 00:01
CRDT- 1998/11/03 00:00
PHST- 1998/11/03 00:00 [pubmed]
PHST- 1998/11/03 00:01 [medline]
PHST- 1998/11/03 00:00 [entrez]
AID - S0165460898000703 [pii]
AID - 10.1016/s0165-4608(98)00070-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Oct 15;106(2):159-65. doi: 
      10.1016/s0165-4608(98)00070-3.