PMID- 9798980
OWN - NLM
STAT- MEDLINE
DCOM- 19990104
LR  - 20061115
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 80
IP  - 4
DP  - 1998 Oct
TI  - Characterization of beta2-glycoprotein I binding to phospholipid membranes.
PG  - 610-4
AB  - The plasma protein beta2-glycoprotein I (beta2-GPI) is a major target of 
      autoantibodies in patients with the antiphospholipid syndrome. To understand the 
      physiological function of beta2-GPI and its potential role in the pathophysiology 
      of the antiphospholipid syndrome, the binding of beta2-GPI to phospholipid 
      membranes was characterized. The interaction of beta2-GPI with unilamellar 
      vesicles containing varying amounts of acidic phospholipids with 
      phosphatidylcholine (PC) was measured at equilibrium via relative light 
      scattering. Analysis of binding isotherms gave apparent Kd values ranging from 
      approximately 5.0 to 0.5 microM over a range of 5-20 mol % anionic phospholipid. 
      Inhibition of binding by increasing ionic strength and Ca2+ ions suggests that 
      binding is primarily electrostatic. These data indicate that beta2-GPI binding to 
      membranes with physiological anionic phospholipid content is relatively weak in 
      comparison to plasma coagulation proteins, suggesting that beta2-GPI does not 
      function as a physiological anticoagulant based on its phospholipid-binding 
      properties.
FAU - Harper, M F
AU  - Harper MF
AD  - Department of Medicine, The University of North Carolina at Chapel Hill, 
      27599-7280, USA.
FAU - Hayes, P M
AU  - Hayes PM
FAU - Lentz, B R
AU  - Lentz BR
FAU - Roubey, R A
AU  - Roubey RA
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Glycoproteins)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Phospholipids)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Glycoproteins/*chemistry
MH  - Humans
MH  - Membranes, Artificial
MH  - Phospholipids/*chemistry
MH  - Protein Binding
MH  - beta 2-Glycoprotein I
EDAT- 1998/11/03 00:00
MHDA- 1998/11/03 00:01
CRDT- 1998/11/03 00:00
PHST- 1998/11/03 00:00 [pubmed]
PHST- 1998/11/03 00:01 [medline]
PHST- 1998/11/03 00:00 [entrez]
AID - 98100610 [pii]
PST - ppublish
SO  - Thromb Haemost. 1998 Oct;80(4):610-4.