PMID- 9800323 OWN - NLM STAT- MEDLINE DCOM- 19981207 LR - 20190915 IS - 0268-9146 (Print) IS - 0268-9146 (Linking) VI - 29 IP - 5 DP - 1998 Oct TI - Integration of chicken cytogenetic and genetic maps: 18 new polymorphic markers isolated from BAC and PAC clones. PG - 348-55 AB - As an approach to integrate the chicken genetic and cytogenetic maps, bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) clones were localized by fluorescence in situ hybridization (FISH) on chromosomes and by genetic mapping on the East Lansing and Compton reference families. Some of the clones used in this study were previously selected for the presence of potentially polymorphic (CA)n repeats and a microsatellite marker was developed when possible for genetic mapping. For other clones, a single strand conformational polymorphism (SSCP) was developed and used for this purpose. Between the two approaches, 18 markers linking the cytogenetic and genetic maps, seven on macrochromosomes and 11 on microchromosomes, were generated. Our results enabled the assignment and orientation of a linkage group to chromosome 3, together with the assignment of linkage groups to eight different microchromosomes, a fraction of the genome lacking mapping data and for which the degree of coverage by the genetic map was not well estimated previously. FAU - Morisson, M AU - Morisson M AD - Laboratoire de Genetique Cellulaire, INRA, Tolosan, France. FAU - Pitel, F AU - Pitel F FAU - Fillon, V AU - Fillon V FAU - Pouzadoux, A AU - Pouzadoux A FAU - Berge, R AU - Berge R FAU - Vit, J P AU - Vit JP FAU - Zoorob, R AU - Zoorob R FAU - Auffray, C AU - Auffray C FAU - Gellin, J AU - Gellin J FAU - Vignal, A AU - Vignal A LA - eng SI - GENBANK/AJ001455 SI - GENBANK/AJ001456 SI - GENBANK/AJ001457 SI - GENBANK/AJ001458 SI - GENBANK/AJ001459 SI - GENBANK/AJ001460 SI - GENBANK/AJ001461 SI - GENBANK/AJ001462 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Anim Genet JT - Animal genetics JID - 8605704 RN - 0 (DNA Primers) RN - 0 (Genetic Markers) SB - IM MH - Animals MH - Base Sequence MH - Chickens/*genetics MH - *Chromosome Mapping MH - Cloning, Molecular MH - Cytogenetics MH - DNA Primers/genetics MH - Genetic Linkage MH - *Genetic Markers MH - Genomic Library MH - In Situ Hybridization, Fluorescence MH - Microsatellite Repeats MH - Polymorphism, Genetic MH - Polymorphism, Single-Stranded Conformational EDAT- 1998/11/04 00:00 MHDA- 1998/11/04 00:01 CRDT- 1998/11/04 00:00 PHST- 1998/11/04 00:00 [pubmed] PHST- 1998/11/04 00:01 [medline] PHST- 1998/11/04 00:00 [entrez] AID - 10.1046/j.1365-2052.1998.295348.x [doi] PST - ppublish SO - Anim Genet. 1998 Oct;29(5):348-55. doi: 10.1046/j.1365-2052.1998.295348.x.