PMID- 9800660
OWN - NLM
STAT- MEDLINE
DCOM- 19990113
LR  - 20190815
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 17
IP  - 8
DP  - 1998 Sep
TI  - Quantitation of acetazolamide in rat plasma, brain tissue and cerebrospinal fluid 
      by high-performance liquid chromatography.
PG  - 1415-21
AB  - A simple and sensitive high-performance liquid chromatographic method for the 
      analysis of acetazolamide (AZ) in rat blood (plasma/serum, whole blood and serum 
      ultrafiltrate), brain tissue and cerebrospinal fluid (CSF) was described. 
      Quantitative extraction of AZ with ethyl acetate from both buffered plasma and 
      brain tissue homogenate (pH 8.0) was achieved. Each extract was evaporated to 
      dryness and the residue was chromatographed on a reversed-phase column. CSF was 
      directly analysed without extraction step. The limits of detection were 0.05 
      microgram ml-1 for plasma, 0.02 microgram g-1 for brain tissue and 0.004 
      microgram ml-1 for CSF. Calibration curves were linear over the working ranges of 
      0.1-100 micrograms ml-1 for plasma, 0.05-50 micrograms g-1 for brain tissue and 
      0.025-50 micrograms ml-1 for CSF. The reproducibility of AZ assay in the rat 
      biologic media indicated very low relative standard deviations (RSDs). The 
      recoveries of AZ added to plasma and brain tissue were more than 96% with an RSD 
      of less than 5%. The present method was applied to studies of plasma 
      concentration profiles of the drug after administration and its distribution into 
      central nervous system.
FAU - Ichikawa, N
AU  - Ichikawa N
AD  - Department of Pharmacy, Shimane Medical University Hospital, Japan.
FAU - Naora, K
AU  - Naora K
FAU - Hirano, H
AU  - Hirano H
FAU - Iwamoto, K
AU  - Iwamoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Carbonic Anhydrase Inhibitors)
RN  - O3FX965V0I (Acetazolamide)
SB  - IM
MH  - Acetazolamide/*analysis/blood/cerebrospinal fluid/pharmacokinetics
MH  - Animals
MH  - *Brain Chemistry
MH  - Carbonic Anhydrase Inhibitors/*analysis/blood/cerebrospinal 
      fluid/pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Injections, Intravenous
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 1998/11/04 00:00
MHDA- 1998/11/04 00:01
CRDT- 1998/11/04 00:00
PHST- 1998/11/04 00:00 [pubmed]
PHST- 1998/11/04 00:01 [medline]
PHST- 1998/11/04 00:00 [entrez]
AID - S0731708598000247 [pii]
AID - 10.1016/s0731-7085(98)00024-7 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 1998 Sep;17(8):1415-21. doi: 10.1016/s0731-7085(98)00024-7.