PMID- 9801755
OWN - NLM
STAT- MEDLINE
DCOM- 19990104
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 20
IP  - 5
DP  - 1998 Oct
TI  - Determination of gene and chromosome dosage in prostatic intraepithelial 
      neoplasia and carcinoma.
PG  - 373-80
AB  - OBJECTIVE: To review the utility of fluorescence in situ hybridization (FISH), 
      polymerase chain reaction (PCR) and comparative genomic hybridization (CGH) in 
      the determination of gene and chromosome dosage in cancer specimens and to 
      discuss the genetic association between prostatic intraepithelial neoplasia (PIN) 
      and adenocarcinoma as detected by these three techniques. STUDY DESIGN: FISH, 
      PCR, and CGH were applied to the same specimens to clarify discrepancies observed 
      by a single technique alone and to further understand prostate carcinogenesis. 
      RESULTS: The most common genetic alterations in PIN and carcinoma were (1) gain 
      of chromosome 7, particularly 7q31; (2) loss of 8p and gain of 8q; and (3) loss 
      of 10q, 16q and 18q. CONCLUSION: Using different techniques on the same specimen 
      was useful to determine genetic relationships between cancer and its precursors. 
      PIN and prostatic carcinoma foci have a similar proportion of genetic changes, 
      but foci of carcinoma usually have more alterations. This supports the hypothesis 
      that PIN is the most likely precursor of prostatic carcinoma.
FAU - Qian, J
AU  - Qian J
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 
      Minnesota 55905, USA.
FAU - Jenkins, R B
AU  - Jenkins RB
FAU - Bostwick, D G
AU  - Bostwick DG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
SB  - IM
MH  - Adenocarcinoma/diagnosis/genetics/pathology
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - *Ploidies
MH  - Polymerase Chain Reaction
MH  - Prostatic Intraepithelial Neoplasia/diagnosis/*genetics/pathology
MH  - Prostatic Neoplasms/diagnosis/*genetics/pathology
RF  - 59
EDAT- 1998/11/05 00:00
MHDA- 1998/11/05 00:01
CRDT- 1998/11/05 00:00
PHST- 1998/11/05 00:00 [pubmed]
PHST- 1998/11/05 00:01 [medline]
PHST- 1998/11/05 00:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 1998 Oct;20(5):373-80.