PMID- 9802375
OWN - NLM
STAT- MEDLINE
DCOM- 19981119
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 102
IP  - 4 Pt 1
DP  - 1998 Oct
TI  - Anti-Fc(episilon)RI auto antibodies and basophil histamine releasability in 
      chronic idiopathic urticaria.
PG  - 651-8
AB  - BACKGROUND: Circulating functional autoantibodies to the high-affinity IgE 
      receptor (Fc(epsilon)RI) or to IgE have been found in approximately one third of 
      patients with chronic idiopathic urticaria (CIU). OBJECTIVE: We sought to compare 
      basophil histamine release and basophil numbers in patients with CIU with and 
      without autoantibodies. METHODS: Basophil histamine release to the 
      anti-Fc(epsilon)RI mAb 22E7, anti-IgE, and formyl-methionyl-leucyl-phenylalanine 
      (fMLP); basophil numbers; and total cellular histamine were measured in 26 
      patients with CIU and 18 healthy control subjects. Twelve patients were 
      classified as having functional anti-Fc(epsilon)RI and/or anti-IgE autoantibodies 
      on the basis of their serum-evoked histamine release from the basophils of 2 
      healthy donors. RESULTS: 22E7 and anti-IgE, but not fMLP, released less histamine 
      from basophils of patients with CIU than from those of control subjects. 
      Mean+/-SEM maximum histamine release to 22E7 from basophils of control subjects 
      and patients with CIU with and without autoantibodies was 38.5%+/-5.0%, 
      17.9%+/-6.0% (P =.01), and 1.0%+/-0.3% (P <.0001), respectively. Similar results 
      were obtained with anti-IgE, which is dependent on and cross-links cell bound 
      IgE, and 22E7, which directly cross-links the IgE receptor. The mean+/-SEM 
      basophil counts for control subjects and patients with CIU without and with 
      autoantibodies were 52+/-7, 34+/-9 (P =.04), and 5+/-1 (P <.0001) x 10(6) 
      cells/L, respectively, and similar changes were found in measurements of total 
      cellular histamine. CONCLUSION: Patients with autoantibodies have both markedly 
      reduced basophil numbers and basophil histamine release to factors acting through 
      Fc(epsilon)RI, which indicates either a residual pool of functionally distinct 
      basophils or may be a consequence of desensitization of the Fc(epsilon)RI 
      pathway.
FAU - Sabroe, R A
AU  - Sabroe RA
AD  - St. John's Institute of Dermatology, UMDS, St. Thomas's Hospital, London, United 
      Kingdom.
FAU - Francis, D M
AU  - Francis DM
FAU - Barr, R M
AU  - Barr RM
FAU - Black, A K
AU  - Black AK
FAU - Greaves, M W
AU  - Greaves MW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Autoantibodies)
RN  - 0 (Receptors, IgE)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Autoantibodies/*biosynthesis/immunology
MH  - Basophils/chemistry/*immunology
MH  - Chronic Disease
MH  - Female
MH  - Histamine/analysis
MH  - *Histamine Release
MH  - Humans
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - Receptors, IgE/*immunology
MH  - Urticaria/*immunology
EDAT- 1998/11/05 00:00
MHDA- 1998/11/05 00:01
CRDT- 1998/11/05 00:00
PHST- 1998/11/05 00:00 [pubmed]
PHST- 1998/11/05 00:01 [medline]
PHST- 1998/11/05 00:00 [entrez]
AID - S0091674998003637 [pii]
AID - 10.1016/s0091-6749(98)70283-0 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 1998 Oct;102(4 Pt 1):651-8. doi: 
      10.1016/s0091-6749(98)70283-0.