PMID- 9803675
OWN - NLM
STAT- MEDLINE
DCOM- 19981201
LR  - 20191024
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 68
IP  - 2
DP  - 1998 Aug
TI  - Induction of antitumor cytotoxic activity using CD34+ cord blood cell-derived and 
      irradiated tumor cell-primed dendritic cells.
PG  - 169-82
AB  - Dendritic cells (DCs) are the most powerful antigen-presenting cells in the 
      immune system. They can activate immunologically naive T cells and improve the 
      efficacy of immunotherapy against tumors. In the present study we investigated 
      whether CD34+ cord blood cell-derived DCs are capable of inducing antitumor 
      cytotoxic cells, such as cytotoxic T lymphocytes (CTLs), NK cells, and monocytes. 
      Cord blood T cells stimulated by DCs pulsed with irradiated K562 major 
      histocompatibility complex (MHC) class I+ cells were highly effective in 
      eliciting a selective killing response against K562 class I+ cells. This killing 
      activity was almost completely abrogated by antibodies to CD8 or MHC class I, but 
      not to CD4. This suggests that tumor cell-pulsed DCs generated from CD34+ cord 
      blood cells are able to induce tumor specific CTLs against corresponding tumor 
      cells from cord blood T cells, and that these CTLs are CD8+ T cells which may 
      recognize tumor cells via MHC class I molecules. This observation has potentially 
      important implications for the use of DCs in clinical immunotherapy in cord blood 
      transplantation.
FAU - Fujii, S
AU  - Fujii S
AD  - Second Department of Internal Medicine, Kumamoto University School of Medicine, 
      Japan.
FAU - Fujimoto, K
AU  - Fujimoto K
FAU - Osato, M
AU  - Osato M
FAU - Matsui, K
AU  - Matsui K
FAU - Takatsuki, K
AU  - Takatsuki K
FAU - Kawakita, M
AU  - Kawakita M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - Antigens, CD34/analysis
MH  - Antigens, Neoplasm/*immunology
MH  - CD8-Positive T-Lymphocytes/physiology
MH  - Cytotoxicity, Immunologic/immunology
MH  - Dendritic Cells/*immunology/radiation effects
MH  - Fetal Blood/*cytology
MH  - Histocompatibility Antigens Class I/analysis
MH  - Humans
MH  - Infant, Newborn
MH  - K562 Cells/immunology
MH  - Monocytes/immunology
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured
EDAT- 1998/11/06 00:00
MHDA- 1998/11/06 00:01
CRDT- 1998/11/06 00:00
PHST- 1998/11/06 00:00 [pubmed]
PHST- 1998/11/06 00:01 [medline]
PHST- 1998/11/06 00:00 [entrez]
AID - S0925571098000693 [pii]
AID - 10.1016/s0925-5710(98)00069-3 [doi]
PST - ppublish
SO  - Int J Hematol. 1998 Aug;68(2):169-82. doi: 10.1016/s0925-5710(98)00069-3.