PMID- 9804459
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20190915
IS  - 1058-2916 (Print)
IS  - 1058-2916 (Linking)
VI  - 44
IP  - 5
DP  - 1998 Sep-Oct
TI  - Comparative studies of glycoprotein Ib in heparin coated and nonheparin coated 
      extracorporeal circulation circuits.
PG  - M397-400
AB  - Contact between blood and artificial materials has various effects on blood. 
      Impairment of platelet function is an especially important and well known effect, 
      but its precise mechanism is not clearly understood. The authors constructed a 
      circulation model to investigate the effect of extracorporeal circulation on 
      platelet membrane glycoproteins (GPs), especially GP Ib, and to compare the 
      changes in GP Ib in heparin coated (group C) and nonheparin coated (group N) 
      circuits. As determined by flow cytometry, GP Ib in both groups decreased on 
      initiating circulation, but the decrease in group N was significantly larger than 
      that in group C. There was no observed change in GP IIb/IIIa levels in either 
      group. The extent of shear stress induced platelet aggregation significantly 
      decreased during circulation in both groups. Decreases in the extent of shear 
      stress induced platelet aggregation were significantly less with the use of 
      heparin coated circuits. In addition, the amount of GP Ib in the high speed 
      pellet decreased progressively during circulation in both groups. In contrast, 
      the amount of GP Ib in the Triton insoluble (low speed) pellet increased 
      dramatically during circulation. However, expression of GP Ib in the Triton 
      soluble platelet fraction was low in both groups. From the results, it was 
      concluded that the cause of the decrease in platelet function during 
      extracorporeal circulation is attributable to the internalization of GP Ib from 
      the platelet surface inside the platelet. It also can be said that a heparin 
      coated circuit is one effective means of controlling this change.
FAU - Hioki, I
AU  - Hioki I
AD  - Department of Thoracic and Cardiovascular Surgery, Mie University School of 
      Medicine, Tsu, Japan.
FAU - Yada, I
AU  - Yada I
FAU - Nishikawa, M
AU  - Nishikawa M
FAU - Shomura, Y
AU  - Shomura Y
FAU - Cruz, B P
AU  - Cruz BP
FAU - Onoda, K
AU  - Onoda K
FAU - Tani, K
AU  - Tani K
FAU - Shimono, T
AU  - Shimono T
FAU - Shinpo, H
AU  - Shinpo H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - ASAIO J
JT  - ASAIO journal (American Society for Artificial Internal Organs : 1992)
JID - 9204109
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Blood Platelets/drug effects/*physiology
MH  - Equipment Design
MH  - Extracorporeal Circulation/*instrumentation
MH  - *Heparin/pharmacology
MH  - Platelet Glycoprotein GPIb-IX Complex/*physiology
EDAT- 1998/11/06 00:00
MHDA- 1998/11/06 00:01
CRDT- 1998/11/06 00:00
PHST- 1998/11/06 00:00 [pubmed]
PHST- 1998/11/06 00:01 [medline]
PHST- 1998/11/06 00:00 [entrez]
AID - 10.1097/00002480-199809000-00014 [doi]
PST - ppublish
SO  - ASAIO J. 1998 Sep-Oct;44(5):M397-400. doi: 10.1097/00002480-199809000-00014.