PMID- 9805880
OWN - NLM
STAT- MEDLINE
DCOM- 19981210
LR  - 20181201
IS  - 0305-7518 (Print)
IS  - 0305-7518 (Linking)
VI  - 69
IP  - 3
DP  - 1998 Sep
TI  - In vitro studies on extracellular matrix production by M.leprae infected murine 
      neurofibroblasts.
PG  - 246-56
AB  - Fibroblasts and a host of macrophage secretory products have been implicated in a 
      number of diseases where excess extracellular matrix (ECM) deposition is the main 
      pathological feature. Fibrosis characterized by excessive deposition of collagen 
      also contributes to the irreversible nerve damage observed in leprosy. Since M. 
      leprae are seen within neurofibroblasts (Nf) in the advanced stages of the 
      disease and macrophages form a common infiltrating cellular constituent of 
      leprous nerves at all stages, secretion of ECM proteins by Nf was studied, in 
      vitro following infection with M. leprae and in the presence of macrophage 
      secretory products. These studies were compared in cells derived from two strains 
      of mice, Swiss White (SW) and C57BL/6, as they differ in their response to M. 
      leprae infection and parallel those observed in lepromatous and tuberculoid 
      patients, respectively. On infection with M. leprae, Nfs showed a decrease in 
      secretion of collagen type IV in SW and type I in C57Bl/6 strain. Macrophages 
      caused a further decrease in the secretion of collagen types affected by M. 
      leprae infection per se, while the other collagen types, viz. I and III in SW 
      strain and III and IV in C57Bl/s strain, were unaffected. This study indicates 
      that neural collagenization in nerves in advanced leprosy may be of Nf origin. 
      However, unlike other diseases with excess collagen deposition, ECM proteins 
      produced by Nfs in response to nerve damage may not be of prime importance in the 
      progression of leprous neuropathy and occur as a general response to loss of 
      cellular content in leprous nerves.
FAU - Singh, N
AU  - Singh N
AD  - Foundation for Medical Research, R.G. Thadani Marg, Bombay, India.
FAU - Birdi, T J
AU  - Birdi TJ
FAU - Chandrashekar, S
AU  - Chandrashekar S
FAU - Antia, N H
AU  - Antia NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lepr Rev
JT  - Leprosy review
JID - 0243711
RN  - 0 (Fibronectins)
RN  - 0 (Proteins)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Extracellular Matrix/*metabolism
MH  - Fibroblasts/*metabolism/microbiology
MH  - Fibronectins/metabolism
MH  - Leprosy/*metabolism/microbiology
MH  - Macrophages, Peritoneal/*metabolism/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mycobacterium leprae/*isolation & purification
MH  - Proteins/metabolism
EDAT- 1998/11/07 00:00
MHDA- 1998/11/07 00:01
CRDT- 1998/11/07 00:00
PHST- 1998/11/07 00:00 [pubmed]
PHST- 1998/11/07 00:01 [medline]
PHST- 1998/11/07 00:00 [entrez]
PST - ppublish
SO  - Lepr Rev. 1998 Sep;69(3):246-56.