PMID- 9806043
OWN - NLM
STAT- MEDLINE
DCOM- 19981120
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 178
IP  - 4
DP  - 1998 Oct
TI  - Exogenous bovine surfactant suppresses tumor necrosis factor-alpha release by 
      murine macrophages stimulated by genital mycoplasmas.
PG  - 1122-5
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that 
      appears to play a significant role in the development of neonatal chronic lung 
      disease (CLD). Inflammation and CLD are also associated with respiratory tract 
      colonization with genital mycoplasmas. The possible protective roles of 
      surfactant in mitigating the inflammatory response to these microbes were 
      investigated. Murine RAW 264.7 macrophages were preincubated with an exogenous 
      surfactant and exposed overnight to sterile media, lipopolysaccharide (LPS), 
      Mycoplasma hominis, or Ureaplasma urealyticum. Macrophages released TNF-alpha in 
      response to challenge with LPS, U. urealyticum, and M. hominis in a 
      concentration-dependent fashion. Surfactant suppressed LPS and M. hominis induced 
      TNF-alpha production in a dose-dependent manner but suppressed U. 
      urealyticum-mediated TNF-alpha production only at the higher dose tested. Similar 
      effects were seen in hyperoxia (95% O2). Thus, exogenous bovine surfactant 
      significantly inhibits the production of TNF-alpha by murine macrophages 
      stimulated with genital mycoplasmas and bacterial LPS.
FAU - Talati, A J
AU  - Talati AJ
AD  - Department of Pediatrics, University of Tennessee, Memphis, USA.
FAU - Crouse, D T
AU  - Crouse DT
FAU - English, B K
AU  - English BK
FAU - Newman, C
AU  - Newman C
FAU - Livingston, L
AU  - Livingston L
FAU - Meals, E
AU  - Meals E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Drug Interactions
MH  - Lipopolysaccharides/pharmacology
MH  - Lung Diseases/immunology/microbiology
MH  - Macrophages/cytology/drug effects/*immunology
MH  - Mice
MH  - Mycoplasma hominis/immunology
MH  - Mycoplasmataceae/*immunology
MH  - Pulmonary Surfactants/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
MH  - Ureaplasma/immunology
EDAT- 1998/11/07 00:00
MHDA- 1998/11/07 00:01
CRDT- 1998/11/07 00:00
PHST- 1998/11/07 00:00 [pubmed]
PHST- 1998/11/07 00:01 [medline]
PHST- 1998/11/07 00:00 [entrez]
AID - 10.1086/515695 [doi]
PST - ppublish
SO  - J Infect Dis. 1998 Oct;178(4):1122-5. doi: 10.1086/515695.