PMID- 9806366
OWN - NLM
STAT- MEDLINE
DCOM- 19990106
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 92
IP  - 3
DP  - 1998 Nov 1
TI  - A modified functional global test to measure protein C, protein S activities and 
      the activated protein C-resistance phenotype.
PG  - 141-8
AB  - Identifying a defect affecting the protein C/protein S (PC/PS) anticoagulant 
      system, using a single global test, has recently become possible thanks to a new 
      methodological approach based on the activation of endogenous plasma PC by 
      Protac, derived from Agkistrodon Contortix snake venom (ACV). The introduction of 
      a commercial test (ProC Global), ACV-based, provides a useful tool for the 
      screening of thrombotic patients since the most frequent causes of inherited 
      thrombophilia are found in the PC/PS system. The test provides information only 
      on the global activity of the anticoagulant pathway but not on PC and PS activity 
      or on the factor V related conditions (e.g., FV Leiden). The present study shows 
      that by carrying out the test alternating the presence of PC-, PS-, or 
      FV-deficient plasma and using appropriate amounts of ACV, it is possible to 
      increase the specificity of the test to correctly evaluate respectively the PC or 
      PS activities or the activated protein C resistance condition (APC-R). These 
      simple modifications applied to the original commercial test allow to detect 
      exactly, using a single, basic methodology, the principal defects affecting the 
      PC/PS anticoagulant pathway. Furthermore, carrying out the tests on an automated 
      coagulometer, in combination or not with the classic ProC Global assay, it is 
      possible to use a unique reagent profile to simultaneously investigate in the 
      same or different samples, the PC, PS, and APC-R defect.
FAU - Gemmati, D
AU  - Gemmati D
AD  - Centre for the Study of Haemostasis and Thrombosis, University of Ferrara, Italy.
FAU - Serino, M L
AU  - Serino ML
FAU - Scapoli, G L
AU  - Scapoli GL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Agkistrodon venoms)
RN  - 0 (Crotalid Venoms)
RN  - 0 (Protein C)
RN  - 0 (Protein S)
RN  - 0 (factor V Leiden)
RN  - 9001-24-5 (Factor V)
SB  - IM
MH  - Activated Protein C Resistance/*metabolism
MH  - Adult
MH  - Blood Coagulation Tests/*methods
MH  - Crotalid Venoms
MH  - Factor V/analysis
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Partial Thromboplastin Time
MH  - Phenotype
MH  - Protein C/*metabolism
MH  - Protein S/*metabolism
EDAT- 1998/11/07 00:00
MHDA- 1998/11/07 00:01
CRDT- 1998/11/07 00:00
PHST- 1998/11/07 00:00 [pubmed]
PHST- 1998/11/07 00:01 [medline]
PHST- 1998/11/07 00:00 [entrez]
AID - S0049-3848(98)00120-0 [pii]
AID - 10.1016/s0049-3848(98)00120-0 [doi]
PST - ppublish
SO  - Thromb Res. 1998 Nov 1;92(3):141-8. doi: 10.1016/s0049-3848(98)00120-0.