PMID- 9806578
OWN - NLM
STAT- MEDLINE
DCOM- 19981117
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 70
IP  - 5
DP  - 1998 Nov
TI  - Ultrarapid detection of sex chromosomes with the use of fluorescence in situ 
      hybridization with direct label DNA probes in single human blastomeres, 
      spermatozoa, amniocytes, and lymphocytes.
PG  - 927-32
AB  - OBJECTIVE: To assess the ultrarapid fluorescence in situ hybridization (FISH) 
      procedure with a 1-minute hybridization time for gender determination. DESIGN: 
      Fluorescence in situ hybridization with direct label fluorescence DNA probes for 
      chromosomes X and Y were tested with the use of different hybridization times and 
      different cell types. SETTING: Hospital-based IVF program. INTERVENTION(S): The 
      efficiency of the FISH procedure with different hybridization times was compared 
      with the use of male lymphocytes. The same FISH procedure, but with only 1-minute 
      hybridization, was carried out in human blastomeres, spermatozoa, uncultured 
      amniocytes, male lymphocytes, and female lymphocytes. MAIN OUTCOME MEASURE(S): 
      Percentages of nuclei with positive signals. RESULT(S): The percentages of nuclei 
      with positive signals in lymphocytes with hybridization times of 1, 3, 4, 10, 30, 
      and 45 minutes were 97%, 97%, 98%, 98%, 98%, and 98%, respectively. The 
      percentages of nuclei with positive signals after FISH with a 1-minute 
      hybridization time in single blastomeres, spermatozoa, amniocytes, male 
      lymphocytes, and female lymphocytes were 94%, 96%, 96%, 98%, and 97%, 
      respectively. CONCLUSION(S): Chromosomes X and Y of human blastomeres. 
      spermatozoa, uncultured amniocytes, and lymphocytes can be detected rapidly with 
      the use of this ultrarapid FISH procedure with a 1-minute hybridization time.
FAU - Liu, J
AU  - Liu J
AD  - The Greater Baltimore Medical Center Fertility Center, Greater Baltimore Medical 
      Center, Maryland 21204, USA. jliu@gbmc.org
FAU - Zheng, X Z
AU  - Zheng XZ
FAU - Baramki, T A
AU  - Baramki TA
FAU - Yazigi, R A
AU  - Yazigi RA
FAU - Compton, G
AU  - Compton G
FAU - Katz, E
AU  - Katz E
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (DNA Probes)
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - Blastomeres/*physiology
MH  - DNA Probes
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*physiology
MH  - Male
MH  - *Sex Chromosomes
MH  - Spermatozoa/*physiology
MH  - Time Factors
EDAT- 1998/11/07 00:00
MHDA- 1998/11/07 00:01
CRDT- 1998/11/07 00:00
PHST- 1998/11/07 00:00 [pubmed]
PHST- 1998/11/07 00:01 [medline]
PHST- 1998/11/07 00:00 [entrez]
AID - S0015-0282(98)00288-X [pii]
AID - 10.1016/s0015-0282(98)00288-x [doi]
PST - ppublish
SO  - Fertil Steril. 1998 Nov;70(5):927-32. doi: 10.1016/s0015-0282(98)00288-x.