PMID- 9806591
OWN - NLM
STAT- MEDLINE
DCOM- 19981119
LR  - 20131121
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 150
IP  - 5
DP  - 1998 Nov
TI  - Ionizing radiation stimulates mitochondrial gene expression and activity.
PG  - 505-12
AB  - Radiation-induced gene expression was examined in cells of a radioresistant human 
      glioblastoma cell line, T98G, using RNA fingerprinting by arbitrary primer 
      polymerase chain reaction. Three of the differentially induced transcripts were 
      cloned and identified as the mitochondrially encoded cytochrome c oxidase (MTCO) 
      subunits 1 and 2, and NADH dehydrogenase subunit 4. The levels of all three mRNAs 
      were increased 1 h after irradiation, with elevated expression persisting for at 
      least 24 h. Similar to radiation, other oxidants lead to induction of MTCO1, an 
      effect which could be prevented by the antioxidant pyrrolidine dithiocarbamate. 
      These results indicate that the increase in mitochondrial gene expression is 
      mediated by oxidative stress. Mitochondria could be a target of signaling by 
      ionizing radiation and oxidants since it is known to be at the site of cellular 
      oxidative stress. The proteins encoded by MTCO1 and other mitochondrial mRNAs 
      characterized are part of the mitochondrial respiratory chain which produces 
      adenosine triphosphate through the process of oxidative phosphorylation. 
      Adenosine triphosphate levels and the mitochondrial membrane potential were found 
      to be increased significantly after irradiation, while mitochondrial mass and 
      mitochondrial DNA levels were unaffected. These data demonstrate the specificity 
      of changes in mitochondrial activity and gene expression after irradiation.
FAU - Gong, B
AU  - Gong B
AD  - Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic 
      Foundation, Ohio 44195, USA.
FAU - Chen, Q
AU  - Chen Q
FAU - Almasan, A
AU  - Almasan A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/biosynthesis/metabolism
MH  - Base Sequence
MH  - Blotting, Northern
MH  - DNA Primers
MH  - *Gamma Rays
MH  - Gene Expression Regulation/*radiation effects
MH  - Humans
MH  - Membrane Potentials
MH  - Mitochondria/metabolism/*radiation effects
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Signal Transduction/radiation effects
MH  - Tumor Cells, Cultured
EDAT- 1998/11/07 00:00
MHDA- 1998/11/07 00:01
CRDT- 1998/11/07 00:00
PHST- 1998/11/07 00:00 [pubmed]
PHST- 1998/11/07 00:01 [medline]
PHST- 1998/11/07 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1998 Nov;150(5):505-12.