PMID- 9806841
OWN - NLM
STAT- MEDLINE
DCOM- 19990107
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 54
IP  - 1
DP  - 1998 Nov 15
TI  - Identification and chromosomal localization of CTNNAL1, a novel protein 
      homologous to alpha-catenin.
PG  - 149-54
AB  - Sodium butyrate (NaB) was shown to induce growth inhibition and apoptosis in a 
      human pancreatic cancer cell line (AsPC-1). To identify the genes that are 
      differentially regulated by NaB, we generated subtracted EST libraries highly 
      enriched for up- or down-regulated transcripts using the suppression subtraction 
      hybridization technique. One of the ESTs identified in the down-regulated library 
      showed significant homology to human alpha-catenin. A cDNA of 2.45 kb that codes 
      for a putative protein of 734 aa was cloned. The cloned cDNA was used as a 
      template in an in vitro transcription-translation reaction. This produced a 
      protein product of about 82 kDa in size. The gene designated as CTNNAL1 (catenin 
      (cadherin-associated protein), alpha-like 1) was found to be ubiquitously 
      expressed in many tissues including pancreas, heart, and skeletal muscle. A human 
      BAC clone containing the gene was isolated and used as a probe for fluorescence 
      in situ hybridization (FISH). Both radiation hybrid and FISH analysis mapped the 
      gene to chromosome band 9q31.2, a region where frequent abnormalities have been 
      observed in bladder carcinoma, esophageal cancers, and several other tumors.
CI  - Copyright 1998 Academic Press.
FAU - Zhang, J S
AU  - Zhang JS
AD  - Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester, 
      Minnesota, 55905, USA.
FAU - Nelson, M
AU  - Nelson M
FAU - Wang, L
AU  - Wang L
FAU - Liu, W
AU  - Liu W
FAU - Qian, C P
AU  - Qian CP
FAU - Shridhar, V
AU  - Shridhar V
FAU - Urrutia, R
AU  - Urrutia R
FAU - Smith, D I
AU  - Smith DI
LA  - eng
SI  - GENBANK/AF030233
GR  - CA48031/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Butyrates)
RN  - 0 (CTNNA1 protein, human)
RN  - 0 (CTNNAL1 protein, human)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (alpha Catenin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Butyrates/pharmacology
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/chemistry/*genetics/metabolism
MH  - DNA, Complementary
MH  - Expressed Sequence Tags
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Pancreatic Neoplasms
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - alpha Catenin
EDAT- 1998/11/10 00:00
MHDA- 1998/11/10 00:01
CRDT- 1998/11/10 00:00
PHST- 1998/11/10 00:00 [pubmed]
PHST- 1998/11/10 00:01 [medline]
PHST- 1998/11/10 00:00 [entrez]
AID - S0888-7543(98)95458-1 [pii]
AID - 10.1006/geno.1998.5458 [doi]
PST - ppublish
SO  - Genomics. 1998 Nov 15;54(1):149-54. doi: 10.1006/geno.1998.5458.