PMID- 9807648
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20190815
IS  - 0006-3223 (Print)
IS  - 0006-3223 (Linking)
VI  - 44
IP  - 9
DP  - 1998 Nov 1
TI  - Inhibition of anxiety in rats by antisense to cholecystokinin precursor protein.
PG  - 915-7
AB  - BACKGROUND: Cholecystokinin (CCK) and its analogs generates anxiety in humans and 
      measurable anxiety-like behaviors in rats. CCK receptor blockers have had mixed 
      results as a treatment approach for anxiety disorders. Since CCK is a peptide, we 
      explored another strategy to reduce CCK levels in brain by antisense 
      oligodeoxynucleotide inhibition of DNA transcription or messenger RNA (mRNA) 
      translation for CCK precursor protein. METHODS: Antisense oligodeoxynucleotide 
      complementary to the start coding region of rat CCK-precursor was 
      intracerebroventricularly (icv) infused into rats three times at 24-hour 
      intervals. Control groups received infusions of either a scramble sequence 
      oligodeoxynucleotide or vehicle. On the fourth day, rats were assessed in the 
      elevated plus maze paradigm. RESULTS: Compared to vehicle and scramble sequence 
      oligodeoxynucleotide control, icv CCK-antisense exogenous administration for 3 
      days significantly diminished anxiety behavior in rats. CONCLUSIONS: Antisense 
      inhibition of CCK-mediated anxiety could have therapeutic potential in human 
      anxiety disorders.
FAU - Cohen, H
AU  - Cohen H
AD  - Ministry of Health Mental Health Center, Faculty of Health Sciences, Anxiety and 
      Stress Research Unit, Ben Gurion University of the Negev, Beer-Sheva, Israel.
FAU - Kaplan, Z
AU  - Kaplan Z
FAU - Kotler, M
AU  - Kotler M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Psychiatry
JT  - Biological psychiatry
JID - 0213264
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (Protein Precursors)
RN  - 78206-77-6 (procholecystokinin)
RN  - 9011-97-6 (Cholecystokinin)
SB  - IM
MH  - Animals
MH  - Anxiety/*drug therapy/physiopathology
MH  - Cholecystokinin/*drug effects/physiology
MH  - Drug Administration Schedule
MH  - Exploratory Behavior/drug effects
MH  - Injections, Intravenous
MH  - Male
MH  - Maze Learning/drug effects
MH  - Oligodeoxyribonucleotides, Antisense/*pharmacology
MH  - Protein Precursors/*drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1998/11/10 00:00
MHDA- 1998/11/10 00:01
CRDT- 1998/11/10 00:00
PHST- 1998/11/10 00:00 [pubmed]
PHST- 1998/11/10 00:01 [medline]
PHST- 1998/11/10 00:00 [entrez]
AID - S0006-3223(98)00010-9 [pii]
AID - 10.1016/s0006-3223(98)00010-9 [doi]
PST - ppublish
SO  - Biol Psychiatry. 1998 Nov 1;44(9):915-7. doi: 10.1016/s0006-3223(98)00010-9.