PMID- 9809035
OWN - NLM
STAT- MEDLINE
DCOM- 19981113
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 107
IP  - 1
DP  - 1998 Nov
TI  - Chromosome 7 abnormalities in prostate cancer detected by dual-color fluorescence 
      in situ hybridization.
PG  - 51-60
AB  - Aneusomy of chromosome 7 and loss at 7q (especially 7q31.1) have been reported in 
      prostate cancer. To further investigate abnormalities of 7q and the relationship 
      with whole chromosome 7 changes, we have conducted a dual-color fluorescence in 
      situ hybridization (FISH) analysis on isolated nuclei from 28 primary prostate 
      cancers. A pericentromeric probe for chromosome 7, five newly isolated 
      sequence-specific bacterial artificial chromosome (BAC) probes from 7q31.1, and 
      one BAC for the epidermal growth factor receptor (EGFR) gene at 7p12 were used in 
      dual color hybridizations. Pericentromeric probes for chromosomes X and 4 were 
      also used as controls. Sixteen (57.1%) of the 28 tumors showed clonal 
      aberrations. Nine of them were trisomy 7 and four were hypertetrasomy for 
      chromosome 7. Deletions at 7q31.1 were found in two of the high grade tumors. 
      With the exception of these two cases, all other cases showed concordant results 
      using all probes. These findings confirm previous studies that aneusomy of 7 is 
      associated with prostate cancer progression, and there may be a tumor suppressor 
      gene (TSG) at 7q31.1 which is associated with tumor progression. In addition, our 
      study indicates: (1) the deletion pattern of individual nuclei infers that 
      deletions at 7q31.1 precede reduplications of chromosome 7; and (2) the 
      amplification of EGFR was not detected at the DNA level, suggesting that 
      activation of this oncogene may play a minor role in prostate cancer.
FAU - Cui, J
AU  - Cui J
AD  - Department of Pediatrics and Human Genetics, University of Utah Health Science 
      Center, Salt Lake City 84112, USA.
FAU - Deubler, D A
AU  - Deubler DA
FAU - Rohr, L R
AU  - Rohr LR
FAU - Zhu, X L
AU  - Zhu XL
FAU - Maxwell, T M
AU  - Maxwell TM
FAU - Changus, J E
AU  - Changus JE
FAU - Brothman, A R
AU  - Brothman AR
LA  - eng
GR  - R01CA46269/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Aneuploidy
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Paraffin Embedding
MH  - Prostatic Neoplasms/*genetics
MH  - X Chromosome/genetics
EDAT- 1998/11/11 00:00
MHDA- 1998/11/11 00:01
CRDT- 1998/11/11 00:00
PHST- 1998/11/11 00:00 [pubmed]
PHST- 1998/11/11 00:01 [medline]
PHST- 1998/11/11 00:00 [entrez]
AID - S0165460898000740 [pii]
AID - 10.1016/s0165-4608(98)00074-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Nov;107(1):51-60. doi: 
      10.1016/s0165-4608(98)00074-0.