PMID- 9811217
OWN - NLM
STAT- MEDLINE
DCOM- 19990107
LR  - 20190131
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 252
IP  - 3
DP  - 1998 Nov
TI  - Immunohistochemical and enzyme-histochemical study on the accessory olfactory 
      bulb of the dog.
PG  - 393-402
AB  - The accessory olfactory bulb (AOB) is a primary center of the vomeronasal system. 
      In the dog, the position and morphology of the AOB remained vague for a long 
      time. Recently, the morphological characteristics of the dog AOB were 
      demonstrated by means of lectin-histochemical, histological, and 
      immunohistochemical staining, although the distribution of each kind of neuron, 
      especially granule cells, remains controversial in the dog AOB. In the present 
      study, we examined the distribution of neuronal elements in the dog AOB by means 
      of immunohistochemical and enzyme-histochemical staining. Horizontal paraffin or 
      frozen sections of the dog AOB were immunostained with antisera against protein 
      gene product 9.5 (PGP 9.5), brain nitric oxide synthase (NOS), glutamic acid 
      decarboxylase (GAD), tyrosine hydroxylase (TH), substance P (SP), and vasoactive 
      intestinal polypeptide (VIP) by avidin-biotin peroxidase complex method. In 
      addition, frozen sections were stained enzyme-histochemically for 
      NADPH-diaphorase. In the dog AOB, vomeronasal nerve fibers, glomeruli, and 
      mitral/tufted cells were PGP 9.5-immunopositive. Mitral/tufted cells were 
      observed in the glomerular layer (GL) and the neuronal cell layer (NCL). In the 
      NCL, a small number of NOS-, GAD-, and SP-immunopositive and NADPH-diaphorase 
      positive granule cells were observed. In the GL, GAD-, TH-, and 
      VIP-immunopositive periglomerular cells were observed. In the GL and the NCL, 
      TH-, and VIP-immunopositive short axon cells were also observed. In addition to 
      these neurons, TH- and SP-immunopositive afferent fibers were observed in the GL 
      and the NCL. We could distinctly demonstrate the distribution of neuronal 
      elements in the dog AOB. Since only a small number of granule cells were present 
      in the dog AOB, the dog AOB did not display such a well-developed GCL as observed 
      in the other mammals.
FAU - Nakajima, T
AU  - Nakajima T
AD  - Department of Veterinary Anatomy, Faculty of Agriculture, Iwate University, 
      Morioka, Japan.
FAU - Sakaue, M
AU  - Sakaue M
FAU - Kato, M
AU  - Kato M
FAU - Saito, S
AU  - Saito S
FAU - Ogawa, K
AU  - Ogawa K
FAU - Taniguchi, K
AU  - Taniguchi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Antibodies)
RN  - 0 (Nerve Tissue Proteins)
RN  - 33507-63-0 (Substance P)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Dogs
MH  - Female
MH  - Glutamate Decarboxylase/analysis/immunology
MH  - Immunoenzyme Techniques
MH  - Male
MH  - NADPH Dehydrogenase/analysis/immunology
MH  - Nerve Tissue Proteins/analysis/immunology
MH  - Nitric Oxide Synthase/*analysis/immunology
MH  - Olfactory Bulb/*chemistry/*enzymology
MH  - Substance P/analysis/immunology
MH  - Thiolester Hydrolases/*analysis/immunology
MH  - Tyrosine 3-Monooxygenase/analysis/immunology
MH  - Ubiquitin Thiolesterase
MH  - Vasoactive Intestinal Peptide/analysis/immunology
EDAT- 1998/11/12 03:03
MHDA- 2000/06/20 09:00
CRDT- 1998/11/12 03:03
PHST- 1998/11/12 03:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/11/12 03:03 [entrez]
AID - 10.1002/(SICI)1097-0185(199811)252:3<393::AID-AR7>3.0.CO;2-T [pii]
AID - 10.1002/(SICI)1097-0185(199811)252:3<393::AID-AR7>3.0.CO;2-T [doi]
PST - ppublish
SO  - Anat Rec. 1998 Nov;252(3):393-402. doi: 
      10.1002/(SICI)1097-0185(199811)252:3<393::AID-AR7>3.0.CO;2-T.