PMID- 9811500
OWN - NLM
STAT- MEDLINE
DCOM- 19981223
LR  - 20181201
IS  - 0022-3549 (Print)
IS  - 0022-3549 (Linking)
VI  - 87
IP  - 11
DP  - 1998 Nov
TI  - Delivery of MUC1 mucin peptide by Poly(d,l-lactic-co-glycolic acid) microspheres 
      induces type 1 T helper immune responses.
PG  - 1421-7
AB  - Synthetic peptides corresponding to the variable tandem repeat domain of the 
      cancer-associated antigen MUC1 mucin are candidates for cancer vaccines. In our 
      investigation mice were immunized via subcutaneous injection with 
      poly(d,l-lactic-co-glycolic acid) (PLGA) microspheres containing a MUC1 mucin 
      peptide. It was hypothesized that microencapsulation of the MUC1 mucin peptide 
      would prime for antigen-specific Th1 responses while avoiding the need for 
      traditional adjuvants and carrier proteins. Furthermore, an immunomodulator, 
      monophosphoryl lipid A (MPLA), was incorporated into the peptide-loaded PLGA 
      microspheres based on its ability to enhance Th1 responses. The results revealed 
      T cell specific immune responses. The cytokine secretion profiles of the T cells 
      consisted of high levels of interferon-gamma with undetectable levels of 
      interleukin-4 and interleukin-10. Moreover, incorporation of MPLA in the MUC1 
      peptide-loaded PLGA microspheres resulted in an increase in interferon-gamma 
      production. The antibody response was negative for IgM and IgG in the absence of 
      MPLA; however, in the presence of MPLA antibody production was negative for IgM 
      with a minimal IgG response consisting of IgG2a, IgG2b, and IgG3. Based on the 
      antibody and cytokine profiles, it was concluded that MUC1 mucin peptide-loaded 
      PLGA microspheres are capable of eliciting specific Th1 responses, which may be 
      enhanced through the use of MPLA.
FAU - Newman, K D
AU  - Newman KD
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton 
      Alberta, Canada.
FAU - Sosnowski, D L
AU  - Sosnowski DL
FAU - Kwon, G S
AU  - Kwon GS
FAU - Samuel, J
AU  - Samuel J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pharm Sci
JT  - Journal of pharmaceutical sciences
JID - 2985195R
RN  - 0 (Drug Carriers)
RN  - 0 (MUC1 tandem repeat peptide)
RN  - 0 (Mucin-1)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Drug Carriers
MH  - *Drug Delivery Systems
MH  - Female
MH  - *Lactic Acid
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microspheres
MH  - Molecular Sequence Data
MH  - Mucin-1
MH  - Oligopeptides/*administration & dosage/immunology
MH  - Peptide Fragments
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - T-Lymphocytes, Helper-Inducer/*immunology
EDAT- 1998/11/13 03:02
MHDA- 2000/07/19 11:00
CRDT- 1998/11/13 03:02
PHST- 1998/11/13 03:02 [pubmed]
PHST- 2000/07/19 11:00 [medline]
PHST- 1998/11/13 03:02 [entrez]
AID - S0022-3549(15)50687-4 [pii]
AID - 10.1021/js980070s [doi]
PST - ppublish
SO  - J Pharm Sci. 1998 Nov;87(11):1421-7. doi: 10.1021/js980070s.