PMID- 9811819
OWN - NLM
STAT- MEDLINE
DCOM- 19981216
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 95
IP  - 23
DP  - 1998 Nov 10
TI  - Retinoic acid X receptor in the diploblast, Tripedalia cystophora.
PG  - 13442-7
AB  - Nuclear hormone receptors comprise a characteristic family of transcription 
      factors found in vertebrates, insects and nematodes. Here we show by cDNA and 
      gene cloning that a Cnidarian, Tripedalia cystophora, possesses a retinoid 
      receptor (jRXR) with remarkable homology to vertebrate retinoic acid X receptors 
      (RXRs). Like vertebrate RXRs, jRXR binds 9-cis retinoic acid (Kd = 4 x 10(-10) M) 
      and binds to the DNA sequence, PuGGTCA as a monomer in vitro. jRXR also 
      heterodimerizes with Xenopus TR beta on a thyroid responsive element of a direct 
      repeat separated by 4 bp. A jRXR binding half-site capable of interacting with 
      (His6)jRXR fusion protein was identified in the promoters of three T. cystophora 
      crystallin genes that are expressed highly in the eye lens of this jellyfish. 
      Because crystallin gene expression is regulated by retionoid signaling in 
      vertebrates, the jellyfish crystallin genes are candidate in vivo targets for 
      jRXR. Finally, an antibody prepared against (His6)jRXR showed that full-length 
      jRXR is expressed at all developmental stages of T. cystophora except the 
      ephydra, where a smaller form replaces is. These data show that Cnidaria, a 
      diploblastic phylum ancestral to the triploblastic invertebrate and subsequent 
      vertebrate lineages, already have an RXR suggesting that RXR is an early 
      component of the regulatory mechanisms of metazoa.
FAU - Kostrouch, Z
AU  - Kostrouch Z
AD  - Diabetes Branch, National Institute of Diabetes, and Digestive and Kidney 
      Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Kostrouchova, M
AU  - Kostrouchova M
FAU - Love, W
AU  - Love W
FAU - Jannini, E
AU  - Jannini E
FAU - Piatigorsky, J
AU  - Piatigorsky J
FAU - Rall, J E
AU  - Rall JE
LA  - eng
SI  - GENBANK/AF091121
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cnidaria/genetics/*metabolism
MH  - DNA, Complementary/*genetics/isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Receptors, Retinoic Acid/*genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics
PMC - PMC24838
EDAT- 1998/11/13 00:00
MHDA- 1998/11/13 00:01
PMCR- 1999/05/10
CRDT- 1998/11/13 00:00
PHST- 1998/11/13 00:00 [pubmed]
PHST- 1998/11/13 00:01 [medline]
PHST- 1998/11/13 00:00 [entrez]
PHST- 1999/05/10 00:00 [pmc-release]
AID - 3661 [pii]
AID - 10.1073/pnas.95.23.13442 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13442-7. doi: 
      10.1073/pnas.95.23.13442.