PMID- 9812779
OWN - NLM
STAT- MEDLINE
DCOM- 19990209
LR  - 20191101
IS  - 0257-716X (Print)
IS  - 0257-716X (Linking)
VI  - 17
IP  - 4
DP  - 1997
TI  - Expression of monocyte chemoattractant protein-1 in monocytes and effects of 
      native and oxidized very low density lipoproteins.
PG  - 203-5
AB  - Monocyte chemoattractant protein-1 (MCP-1), a potent chemoattractant, is thought 
      to play an important role in migration of monocytes into atherosclerotic lesions. 
      The present study was designed to investigate the capacity of human peripheral 
      blood monocytes to express MCP-1 and effects of native very low density 
      lipoprotein (VLDL) and oxidized VLDL (OX-VLDL) on the expression. The total RNA 
      was extracted from cultured monocytes, which were exposed to VLDL and OX-VLDL, 
      and the media conditioned by monocytes were collected. MCP-1 mRNA expression was 
      examined by Northern blot analysis. MCP-1 protein in conditioned media was 
      determined by using sandwich ELISA. The results showed that monocytes can express 
      MCP-1 after a 24 h incubation at 37 degrees C, and the expression was markedly 
      increased by a exposure to OX-VLDL, whereas the expression was slightly increased 
      when exposed to VLDL. It suggests that the capacity of monocytes to produce MCP-1 
      that recruits and activates circulating monocytes may be of considerable 
      importance in atherogenesis, and oxidation of VLDL enhances its potential to 
      promote atherogenesis.
FAU - Wang, G
AU  - Wang G
AD  - Department of Pathology, Tongji Medical University, Wuhan.
FAU - Deng, Z
AU  - Deng Z
FAU - Ni, J
AU  - Ni J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tongji Med Univ
JT  - Journal of Tongji Medical University = Tong ji yi ke da xue xue bao
JID - 8605495
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Arteriosclerosis/etiology/metabolism
MH  - Chemokine CCL2/*biosynthesis
MH  - Humans
MH  - Lipoproteins, VLDL/*metabolism
MH  - Monocytes/*metabolism
MH  - Oxidation-Reduction
MH  - RNA, Messenger/biosynthesis
EDAT- 1997/01/01 00:00
MHDA- 1998/11/13 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/11/13 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/BF02895619 [doi]
PST - ppublish
SO  - J Tongji Med Univ. 1997;17(4):203-5. doi: 10.1007/BF02895619.