PMID- 9812835
OWN - NLM
STAT- MEDLINE
DCOM- 19981217
LR  - 20131121
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 49
IP  - 1
DP  - 1997 Feb
TI  - [L-arginine transport in cultured vascular smooth muscle cells of spontaneously 
      hypertensive rats and effect of liposome on the transport].
PG  - 67-72
AB  - The characteristics of L-arginine (L-Arg) transport in cultured aortic smooth 
      muscle cells (SMC) of spontaneously hypertensive rat (SHR) and control WKY rats 
      were studied and the effect of liposome as L-Arg carrier on the transport was 
      investigated. The results showed that the L-Arg transport of SMC in SHR was 
      obviously lower than that in WKY rats. Maximum transport velocity (Vmax) of high 
      and low affinity in SHR were respectively 48% (P < 0.01) and 49% (P < 0.01) of 
      WKY rat, while the michaelis constant (K(m)) showed no significant difference (P 
      > 0.05). Increase of L-Arg transport induced by tumor necrosis factor-alpha (TNF 
      alpha) in SMC of SHR was obviously lower than that in WKY rats (P < 0.01). The 
      uptake of L-Arg increased 10 to 20 times in SMC when incubated with liposome 
      encapsulated L-Arg (Liposome-L-Arg) than with free L-Arg. The transport velocity 
      in SMC incubated with liposome-L-Arg showed no significant difference in SHR and 
      WKY rats (P > 0.05). The transport of liposome-L-Arg in SMC was not affected by 
      TNF alpha in both the types of rats. The above results indicate that there exists 
      a functional disturbance in L-Arg transport in the SMC of SHR, but the L-Arg 
      transport in SMC can be obviously enhanced when liposome is used as L-Arg 
      carrier. Thus, it appears that liposome-L-Arg may have clinical perspective in 
      the treatment of hypertension.
FAU - Yao, X H
AU  - Yao XH
AD  - Institute of Cardiovascular Basic Research, Beijing Medical University.
FAU - Lu, X Y
AU  - Lu XY
FAU - Zhang, L Z
AU  - Zhang LZ
FAU - Niu, D D
AU  - Niu DD
FAU - Gao, L R
AU  - Gao LR
FAU - Pang, Y Z
AU  - Pang YZ
FAU - Su, J Y
AU  - Su JY
FAU - Tang, C S
AU  - Tang CS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Drug Carriers)
RN  - 0 (Liposomes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/metabolism
MH  - Arginine/*metabolism
MH  - Biological Transport
MH  - Cells, Cultured
MH  - Drug Carriers
MH  - Hypertension/*metabolism
MH  - Liposomes
MH  - Muscle, Smooth, Vascular/cytology/*metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1997/02/01 00:00
MHDA- 1998/11/13 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1998/11/13 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 1997 Feb;49(1):67-72.