PMID- 9814665
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20191102
IS  - 0961-2033 (Print)
IS  - 0961-2033 (Linking)
VI  - 7 Suppl 2
DP  - 1998
TI  - Epitopes on beta2-GPI recognized by anticardiolipin antibodies.
PG  - S14-7
AB  - Anticardiolipin antibodies (aCL) found in sera from patients with 
      antiphospholipid syndrome recognize a cryptic epitope that appears on the 
      beta2-glycoprotein I (beta2-GPI) molecule when beta2-GPI interacts with a lipid 
      membrane composed of negatively charged phospholipid or when beta2-GPI is 
      adsorbed on a polyoxygenated polystyrene plate. A homology based model of 
      beta2-GPI was constructed based on the NMR coordinates of sushi domains of human 
      factor H. The conformation was like a cylinder consisting of five domains, its IV 
      and V domains being glued by electrostatic interaction. We used phage-displayed 
      random peptide libraries to search the epitopes of human aCL. Structures similar 
      to consensus sequences selected by a biopanning method was found on domain IV of 
      beta2-GPI.
FAU - Koike, T
AU  - Koike T
AD  - Department of Medicine II, Hokkaido University School of Medicine, Sapporo, 
      Japan. tkoike@med.hokudai.ac.jp
FAU - Ichikawa, K
AU  - Ichikawa K
FAU - Kasahara, H
AU  - Kasahara H
FAU - Atsumi, T
AU  - Atsumi T
FAU - Tsutsumi, A
AU  - Tsutsumi A
FAU - Matsuura, E
AU  - Matsuura E
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Lupus
JT  - Lupus
JID - 9204265
RN  - 0 (Antibodies, Anticardiolipin)
RN  - 0 (Autoantigens)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Phospholipids)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Anticardiolipin/*immunology
MH  - Antiphospholipid Syndrome/*immunology
MH  - Autoantigens/chemistry/*immunology
MH  - Autoimmune Diseases/*immunology
MH  - Epitopes/*immunology
MH  - Glycoproteins/chemistry/*immunology
MH  - Humans
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phospholipids/immunology/metabolism
MH  - Protein Conformation
MH  - beta 2-Glycoprotein I
RF  - 33
EDAT- 1998/11/14 00:00
MHDA- 1998/11/14 00:01
CRDT- 1998/11/14 00:00
PHST- 1998/11/14 00:00 [pubmed]
PHST- 1998/11/14 00:01 [medline]
PHST- 1998/11/14 00:00 [entrez]
AID - 10.1177/096120339800700204 [doi]
PST - ppublish
SO  - Lupus. 1998;7 Suppl 2:S14-7. doi: 10.1177/096120339800700204.