PMID- 9815245
OWN - NLM
STAT- MEDLINE
DCOM- 19990107
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 178
IP  - 6
DP  - 1998 Dec
TI  - Interleukin-18 enhances lipopolysaccharide-induced interferon-gamma production in 
      human whole blood cultures.
PG  - 1830-4
AB  - Interleukin-18 (IL-18) is a newly described cytokine, formerly called 
      interferon-gamma (IFN-gamma)-inducing factor. In a simple 24-h human whole blood 
      culture, IFN-gamma was produced by the combination of lipopolysaccharide (LPS) 
      plus IL-18. To liberate cytokines in the leukocyte and red cell compartments, the 
      detergent Triton X-100 was added to the entire blood culture. The combination of 
      low concentrations of LPS plus IL-18 induced a 3- to 5-fold greater production of 
      IFN-gamma than did either stimulant alone. Tumor necrosis factor-alpha 
      (TNF-alpha), IL-6, and IL-8 were also produced. The presence of IL-10 completely 
      suppressed the production of IFN-gamma and reduced that of TNF-alpha, IL-6, and 
      IL-8. Thus, IFN-gamma, TNF-alpha, IL-8, and IL-6 are produced in a single whole 
      blood culture, making correlations in the synthesis of a T helper type 1 cytokine 
      and proinflammatory cytokines with disease activity possible in a single culture.
FAU - Puren, A J
AU  - Puren AJ
AD  - Department of Medicine, Division of Infectious Diseases, University of Colorado 
      Health Sciences Center, Denver, Colorado, USA.
FAU - Razeghi, P
AU  - Razeghi P
FAU - Fantuzzi, G
AU  - Fantuzzi G
FAU - Dinarello, C A
AU  - Dinarello CA
LA  - eng
GR  - AI-15614/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Blood Cells/drug effects/*immunology
MH  - Cells, Cultured
MH  - Drug Synergism
MH  - Female
MH  - Humans
MH  - Interferon-gamma/*biosynthesis/blood
MH  - Interleukin-10/pharmacology/physiology
MH  - Interleukin-18/*pharmacology/physiology
MH  - Interleukin-6/biosynthesis/blood
MH  - Interleukin-8/biosynthesis/blood
MH  - Kinetics
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Middle Aged
MH  - Reference Values
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1998/11/17 03:02
MHDA- 2000/03/21 09:00
CRDT- 1998/11/17 03:02
PHST- 1998/11/17 03:02 [pubmed]
PHST- 2000/03/21 09:00 [medline]
PHST- 1998/11/17 03:02 [entrez]
AID - JID971509 [pii]
AID - 10.1086/314481 [doi]
PST - ppublish
SO  - J Infect Dis. 1998 Dec;178(6):1830-4. doi: 10.1086/314481.