PMID- 9816100
OWN - NLM
STAT- MEDLINE
DCOM- 19990210
LR  - 20071114
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 2
IP  - 1
DP  - 1996 Jan
TI  - Aneusomies of chromosomes 8 and Y detected by fluorescence in situ hybridization 
      are prognostic markers for pathological stage C (pt3N0M0) prostate carcinoma.
PG  - 137-45
AB  - In an attempt to identify new prognostic markers, we performed fluorescence in 
      situ hybridization (FISH) ploidy analysis of tumor tissue from patients with a 
      targeted stage and histological grade of prostate carcinoma. We identified all 
      227 patients from the Mayo Clinic radical prostatectomy data base who had a high 
      histological grade pathological stage C (pT3N0M0) tumor removed between 1966 and 
      1987. After histological review of the paraffin-embedded specimen blocks, 181 
      cases were suitable for FISH analysis using chromosome enumeration probes for 
      chromosomes 7, 8, 10, 12, X, and Y. FISH detected 80 (44%) diploid, 22 (12%) 
      tetraploid, and 79 (44%) aneuploid tumors. The common aneusomies were of 
      chromosomes 7 and 8, which were present in 51 (28%) and 46 (25%) tumors, 
      respectively. Aneusomies of chromosomes 10, 12, X, and Y were observed in 11 
      (6%), 15 (8%) 12 (7%) and 16 (9%) tumors, respectively. FISH aneuploid tumors 
      showed a trend of more frequent systemic prostate cancer progression than 
      nonaneuploid tumors (P = 0.060). For individual chromosome anomalies, gains of 
      chromosome 8, aneusomy of chromosome 8, and aneusomy of chromosome Y correlated 
      highly with systemic cancer progression (P = 0.006, 0.013, and 0.021, 
      respectively). Gains of chromosome Y and aneusomy of chromosome Y were associated 
      with an increased prostate cancer death rate (P < 0.001 for both). Multivariate 
      analysis showed that gains of chromosome 8 and aneusomy of chromosome Y were 
      significant independent "predictors" of systemic cancer progression (P = 0.008) 
      and cancer death (P < 0.001), respectively. These results demonstrate that 
      aneuploidy and specific aneusomies detected by FISH are potential markers for a 
      poor prognosis in histological high-grade pathological stage C (pT3N0M0) prostate 
      carcinoma.
FAU - Takahashi, S
AU  - Takahashi S
AD  - Department of Urology and Laboratory Medicine and Pathology and Section of 
      Biostatistics, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
FAU - Alcaraz, A
AU  - Alcaraz A
FAU - Brown, J A
AU  - Brown JA
FAU - Borell, T J
AU  - Borell TJ
FAU - Herath, J F
AU  - Herath JF
FAU - Bergstralh, E J
AU  - Bergstralh EJ
FAU - Lieber, M M
AU  - Lieber MM
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
GR  - P20 CA 58225/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
SB  - IM
MH  - Aged
MH  - *Aneuploidy
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Prostatic Neoplasms/*genetics/mortality/pathology
MH  - Retrospective Studies
MH  - Survival Rate
MH  - *Y Chromosome
EDAT- 1996/01/01 00:00
MHDA- 1998/11/17 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1998/11/17 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1996 Jan;2(1):137-45.