PMID- 9816332
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20151119
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 2
IP  - 9
DP  - 1996 Sep
TI  - Trisomy 7 by dual-color fluorescence in situ hybridization: a potential 
      biological marker for prostate cancer progression.
PG  - 1553-8
AB  - Smear preparations from fine-needle aspirates of 30 prostatic carcinomas obtained 
      from radical prostatectomy specimens were examined by a dual-color fluorescence 
      in situ hybridization (FISH) method for the presence of chromosome 7 trisomy 
      (chromosome 9 was used as a control). The frequency of cells with trisomy 7 was 
      determined in tumor cells and normal prostatic epithelial cells in each specimen. 
      Comparison between the tumor and normal cells from the same patients showed that 
      within all stages, the frequency of trisomy 7/disomy 9 cells in the tumor cells 
      was significantly higher than that observed in the normal cells (P < 0.0001). 
      Furthermore, the mean frequency of cells with trisomy 7/disomy 9 in advanced 
      stages was significantly elevated over the mean frequency observed in 
      organ-confined tumors (P = 0.02). These results are consistent with our previous 
      data on paraffin-embedded prostate tissue sections using single-color FISH 
      procedures. However, the method used in the present study enhances the accuracy 
      of distinguishing trisomic 7 cells from potentially triploid (trisomy 7/trisomy 
      9) cells. Furthermore, the use of fine-needle aspirates rather than paraffin 
      sections provides an easy method to examine whole nuclei. Our study also suggests 
      that FISH provides a better measure of genetic instability (e.g., aneuploidy) in 
      prostate tumors than flow cytometry.
FAU - Wang, R Y
AU  - Wang RY
AD  - Departments of Laboratory Medicine, Pathology, and Biomathematics, The University 
      of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
FAU - Troncoso, P
AU  - Troncoso P
FAU - Palmer, J L
AU  - Palmer JL
FAU - El-Naggar, A K
AU  - El-Naggar AK
FAU - Liang, J C
AU  - Liang JC
LA  - eng
GR  - CA67964/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (Biomarkers)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Biomarkers
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Disease Progression
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Ploidies
MH  - Prostatic Neoplasms/genetics/pathology
MH  - *Trisomy
EDAT- 1996/09/01 00:00
MHDA- 1998/11/17 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1998/11/17 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1996 Sep;2(9):1553-8.