PMID- 9817916
OWN - NLM
STAT- MEDLINE
DCOM- 19990121
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 7
IP  - 13
DP  - 1998 Dec
TI  - Large multi-chromosomal duplications encompass many members of the olfactory 
      receptor gene family in the human genome.
PG  - 2007-20
AB  - The human genome contains thousands of genes that encode a diverse repertoire of 
      odorant receptors (ORs). We report here on the identification and chromosomal 
      localization of 74 OR-containing genomic clones. Using fluorescence in situ 
      hybridization (FISH), we demonstrate a striking homology among a set of 
      approximately 20 OR locations, illustrating a history of duplications that have 
      distributed OR sequences across the genome. Half of the OR-containing BACs cloned 
      from total genomic DNA and 86% of cosmids derived from chromosome 3 
      cross-hybridize to a subset of these locations, many to 17 of them. These 
      paralogous regions are distributed on 13 chromosomes, and eight lie in terminal 
      bands. By analyzing clones from an approximately 250 kb clone-walk across one of 
      these sites (3p13), we show that the homology among these sites is extensive 
      (>150 kb) and encompasses both OR genes and intergenic genomic sequences. The 
      FISH signals appear significantly larger at some sites than at the native 
      location, indicating that portions of some duplicons have undergone local 
      amplification/attrition. More restricted duplications involving pairs of other 
      genomic locations are detected with 12% of the OR-BACs. Only a small subset of OR 
      locations is sufficiently diverged from the others that clones derived from them 
      behave as single-copy FISH probes. We estimate that duplications encompassing 
      members of the OR gene family account for >0.1% of the human genome. A comparison 
      of FISH signals at orthologous locations in other primates indicates that a 
      portion of this OR 'subgenome' has been in flux during the divergence of 
      primates, possibly as a mechanism for evolving the repertoire of olfactory 
      receptors.
FAU - Trask, B J
AU  - Trask BJ
AD  - Department of Molecular Biotechnology, Box 357730, University of Washington, 
      Seattle, WA 98195, USA. trask@biotech.washington.edu
FAU - Massa, H
AU  - Massa H
FAU - Brand-Arpon, V
AU  - Brand-Arpon V
FAU - Chan, K
AU  - Chan K
FAU - Friedman, C
AU  - Friedman C
FAU - Nguyen, O T
AU  - Nguyen OT
FAU - Eichler, E
AU  - Eichler E
FAU - van den Engh, G
AU  - van den Engh G
FAU - Rouquier, S
AU  - Rouquier S
FAU - Shizuya, H
AU  - Shizuya H
FAU - Giorgi, D
AU  - Giorgi D
LA  - eng
GR  - GM57070-01/GM/NIGMS NIH HHS/United States
GR  - HG01464/HG/NHGRI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Receptors, Odorant)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Chromosomes, Human/genetics
MH  - Cloning, Molecular
MH  - *Genome, Human
MH  - Genomic Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Multigene Family/*genetics
MH  - Primates/genetics
MH  - Receptors, Odorant/*genetics
EDAT- 1998/11/18 00:00
MHDA- 1998/11/18 00:01
CRDT- 1998/11/18 00:00
PHST- 1998/11/18 00:00 [pubmed]
PHST- 1998/11/18 00:01 [medline]
PHST- 1998/11/18 00:00 [entrez]
AID - ddb261 [pii]
AID - 10.1093/hmg/7.13.2007 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1998 Dec;7(13):2007-20. doi: 10.1093/hmg/7.13.2007.