PMID- 9819666
OWN - NLM
STAT- MEDLINE
DCOM- 19981214
LR  - 20181201
IS  - 0141-2760 (Print)
IS  - 0141-2760 (Linking)
VI  - 48
IP  - 4
DP  - 1996
TI  - Adjuvant activities of pyrene, anthracene, fluoranthene and benzo(a)pyrene in 
      production of anti-IgE antibody to Japanese cedar pollen allergen in mice.
PG  - 133-47
AB  - We have previously demonstrated that pyrene in diesel-exhaust particles (DEP) has 
      an adjuvant activity on immunoglobulin E (IgE) antibody production in mice 
      immunized with Japanese cedar pollen allergen (JCPA) or ovalbumin (OA) 
      intraperitoneally. The present study is concerned with the adjuvant activity in 
      IgE antibody production against JCPA of pyrene or DEP inoculated intranasally in 
      mice. We show that anthracene, fluoranthene and benzo(a)pyrene in DEP have the 
      ability to enhance anti-JCPA IgE antibody production in mice by intranasal 
      immunization. Mice were grouped, immunized with 10 micrograms of JCPA plus 400 
      micrograms of pyrene, 10 micrograms of JCPA plus 100 micrograms of DEP, 10 
      micrograms of JCPA plus 2 mg of aluminum hydroxide and 10 micrograms of JCPA 
      alone intranasally 7 times at 2 week intervals. Mice were also grouped, and 
      immunized with JCPA (10 micrograms) plus 40 micrograms of anthracene, JCPA (10 
      micrograms) plus 400 micrograms of fluoranthene, JCPA (10 micrograms) plus 40 
      micrograms of benzo(a)pyrene, and JCPA (10 micrograms) plus 400 micrograms of 
      pyrene and JCPA (10 micrograms) alone. We found that the IgE antibody responses 
      to JCPA in mice immunized with JCPA plus pyrene, JCPA plus DEP or JCPA plus the 
      three chemical organic compounds mentioned above were significantly enhanced 
      compared with those immunized with JCPA alone. In addition, when the 
      intraperitoneal macrophages obtained from the normal mice (unimmunized mice) were 
      incubated with pyrene, anthracene, fluoranthene or benzo(a)pyrene in vitro, an 
      enhanced chemiluminescence (CI) response and interleukin-1 alpha (IL-1 alpha) 
      production of the macrophages was observed in each instance. These results 
      suggest that in the production of IgE antibody to JCPA the adjuvancy of 
      polycyclic aromatic hydrocarbons (PAHs) in DEP may be important in an attack of 
      Japanese cedar pollinosis.
FAU - Kanoh, T
AU  - Kanoh T
AD  - Miyazaki Medical College, Japan.
FAU - Suzuki, T
AU  - Suzuki T
FAU - Ishimori, M
AU  - Ishimori M
FAU - Ikeda, S
AU  - Ikeda S
FAU - Ohasawa, M
AU  - Ohasawa M
FAU - Ohkuni, H
AU  - Ohkuni H
FAU - Tunetoshi, Y
AU  - Tunetoshi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Scotland
TA  - J Clin Lab Immunol
JT  - Journal of clinical & laboratory immunology
JID - 7808987
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Air Pollutants)
RN  - 0 (Allergens)
RN  - 0 (Anthracenes)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Fluorenes)
RN  - 0 (Interleukin-1)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Pyrenes)
RN  - 0 (Vehicle Emissions)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 360UOL779Z (fluoranthene)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 5QB0T2IUN0 (Aluminum Hydroxide)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9E0T7WFW93 (pyrene)
RN  - EH46A1TLD7 (anthracene)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Administration, Intranasal
MH  - Air Pollutants/*adverse effects/chemistry/pharmacology
MH  - Allergens/administration & dosage/*immunology
MH  - Aluminum Hydroxide/pharmacology
MH  - Animals
MH  - Anthracenes/pharmacology
MH  - Antibodies, Anti-Idiotypic/*biosynthesis/immunology
MH  - Benzo(a)pyrene/pharmacology
MH  - Female
MH  - Fluorenes/pharmacology
MH  - Food Contamination/analysis
MH  - Humans
MH  - Immunization
MH  - Immunoglobulin E/*immunology
MH  - Interleukin-1/metabolism
MH  - Luminescent Measurements
MH  - Macrophages, Peritoneal/immunology/metabolism
MH  - Male
MH  - Mice
MH  - Ovalbumin/administration & dosage/immunology
MH  - Pollen/adverse effects/*immunology
MH  - Polycyclic Aromatic Hydrocarbons/adverse effects/analysis/*pharmacology
MH  - Pyrenes/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Rhinitis, Allergic, Seasonal/*etiology/immunology
MH  - Trees
MH  - Urban Population
MH  - Vehicle Emissions/*adverse effects
EDAT- 1996/01/01 00:00
MHDA- 1998/11/20 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1998/11/20 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PST - ppublish
SO  - J Clin Lab Immunol. 1996;48(4):133-47.