PMID- 9820161 OWN - NLM STAT- MEDLINE DCOM- 19981207 LR - 20081121 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 79 ( Pt 11) DP - 1998 Nov TI - Types of variation in DNA-A among isolates of East African cassava mosaic virus from Kenya, Malawi and Tanzania. PG - 2835-40 AB - Complete nucleotide sequences of the DNA-A-like molecules of three East African cassava mosaic virus (EACMV) isolates from Kenya (-K, 2801 nt) and Malawi (-MH and -MK, both 2804 nt) were determined. These sequences were compared with that published for a Tanzanian isolate (-T, 2801 nt) and the partial sequence of a third Malawian isolate. Intergenic region sequences of all isolates, and deduced amino acid sequences of their AC1 (Rep) proteins, each formed a tightly related cluster that was distinct from the comparable components of other begomoviruses. Other complementary-sense genes (AC2, AC3, AC4) differed between EACMV isolates in a way consistent with the accumulation of point mutations. In contrast, virus-sense genes (CP, AV2) of isolates -MH and -MK differed (substantially for AV2) from those of other EACMV isolates but somewhat resembled those of tomato yellow leaf curl virus-Israel, suggesting they had been acquired by recombination with an unidentified begomovirus. FAU - Zhou, X AU - Zhou X AD - Scottish Crop Research Institute, Invergowrie, Dundee, UK. FAU - Robinson, D J AU - Robinson DJ FAU - Harrison, B D AU - Harrison BD LA - eng SI - GENBANK/AJ006458 SI - GENBANK/AJ006459 SI - GENBANK/AJ006460 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (DNA, Viral) SB - IM MH - Capsid/genetics MH - DNA, Viral/*genetics MH - *Genetic Variation MH - *Genome, Viral MH - Kenya MH - Malawi MH - Manihot/*virology MH - Molecular Sequence Data MH - Mosaic Viruses/*genetics MH - Phylogeny MH - Sequence Analysis, DNA MH - Tanzania EDAT- 1998/11/20 00:00 MHDA- 1998/11/20 00:01 CRDT- 1998/11/20 00:00 PHST- 1998/11/20 00:00 [pubmed] PHST- 1998/11/20 00:01 [medline] PHST- 1998/11/20 00:00 [entrez] AID - 10.1099/0022-1317-79-11-2835 [doi] PST - ppublish SO - J Gen Virol. 1998 Nov;79 ( Pt 11):2835-40. doi: 10.1099/0022-1317-79-11-2835.